Baculovirus Surface Display of SARS Coronavirus (SARS-CoV) Spike Protein and Immunogenicity of the Displayed Protein in Mice Models

Qian Feng; Yingying Liu; Xiuxia Qu; Hongkui Deng; Mingxiao Ding; Terence L.T. Lau; Albert Cheung-Hoi Yu; Jianguo Chen

doi:10.1089/dna.2006.25.668

Baculovirus Surface Display of SARS Coronavirus (SARS-CoV) Spike Protein and Immunogenicity of the Displayed Protein in Mice Models

Qian Feng, Yingying Liu, Xiuxia Qu, Hongkui Deng, Mingxiao Ding, Terence L.T. Lau, Albert Cheung-Hoi Yu, Jianguo Chen

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Abstract

The baculovirus surface display technique has provided an ideal tool to display foreign proteins with natural conformation, functions, and immunogenicity. In this work, we explored the application of this technique on SARS-associated coronavirus (SARS-CoV) spike (S) protein, and further analyzed the immunogenicity of displayed S protein. The entire ectodomain of S protein was fused between the gp64 signal peptide and the VSV-G membrane anchor and successfully displayed on the baculovirus surface. Subcutaneous injection with purified S-displayed baculoviruses without adjuvant elicited highly effective production of specific and neutralizing antibodies against S protein in mice. These results confirmed a successful surface display of S protein on baculoviruse, and suggested a potential role of S-displayed baculoviruses as a novel live virus-based vaccine candidate for SARS-CoV.

Original language	English
Pages (from-to)	668-673
Number of pages	6
Journal	DNA and Cell Biology
Volume	25
Issue number	12
DOIs	https://doi.org/10.1089/dna.2006.25.668
Publication status	Published - Dec 2006

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title = "Baculovirus Surface Display of SARS Coronavirus (SARS-CoV) Spike Protein and Immunogenicity of the Displayed Protein in Mice Models",

abstract = "The baculovirus surface display technique has provided an ideal tool to display foreign proteins with natural conformation, functions, and immunogenicity. In this work, we explored the application of this technique on SARS-associated coronavirus (SARS-CoV) spike (S) protein, and further analyzed the immunogenicity of displayed S protein. The entire ectodomain of S protein was fused between the gp64 signal peptide and the VSV-G membrane anchor and successfully displayed on the baculovirus surface. Subcutaneous injection with purified S-displayed baculoviruses without adjuvant elicited highly effective production of specific and neutralizing antibodies against S protein in mice. These results confirmed a successful surface display of S protein on baculoviruse, and suggested a potential role of S-displayed baculoviruses as a novel live virus-based vaccine candidate for SARS-CoV.",

author = "Qian Feng and Yingying Liu and Xiuxia Qu and Hongkui Deng and Mingxiao Ding and Lau, {Terence L.T.} and Yu, {Albert Cheung-Hoi} and Jianguo Chen",

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year = "2006",

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doi = "10.1089/dna.2006.25.668",

language = "English",

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T1 - Baculovirus Surface Display of SARS Coronavirus (SARS-CoV) Spike Protein and Immunogenicity of the Displayed Protein in Mice Models

AU - Feng, Qian

AU - Liu, Yingying

AU - Qu, Xiuxia

AU - Deng, Hongkui

AU - Ding, Mingxiao

AU - Lau, Terence L.T.

AU - Yu, Albert Cheung-Hoi

AU - Chen, Jianguo

PY - 2006/12

Y1 - 2006/12

N2 - The baculovirus surface display technique has provided an ideal tool to display foreign proteins with natural conformation, functions, and immunogenicity. In this work, we explored the application of this technique on SARS-associated coronavirus (SARS-CoV) spike (S) protein, and further analyzed the immunogenicity of displayed S protein. The entire ectodomain of S protein was fused between the gp64 signal peptide and the VSV-G membrane anchor and successfully displayed on the baculovirus surface. Subcutaneous injection with purified S-displayed baculoviruses without adjuvant elicited highly effective production of specific and neutralizing antibodies against S protein in mice. These results confirmed a successful surface display of S protein on baculoviruse, and suggested a potential role of S-displayed baculoviruses as a novel live virus-based vaccine candidate for SARS-CoV.

AB - The baculovirus surface display technique has provided an ideal tool to display foreign proteins with natural conformation, functions, and immunogenicity. In this work, we explored the application of this technique on SARS-associated coronavirus (SARS-CoV) spike (S) protein, and further analyzed the immunogenicity of displayed S protein. The entire ectodomain of S protein was fused between the gp64 signal peptide and the VSV-G membrane anchor and successfully displayed on the baculovirus surface. Subcutaneous injection with purified S-displayed baculoviruses without adjuvant elicited highly effective production of specific and neutralizing antibodies against S protein in mice. These results confirmed a successful surface display of S protein on baculoviruse, and suggested a potential role of S-displayed baculoviruses as a novel live virus-based vaccine candidate for SARS-CoV.

U2 - 10.1089/dna.2006.25.668

DO - 10.1089/dna.2006.25.668

M3 - Journal article

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JO - DNA and Cell Biology

JF - DNA and Cell Biology

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ER -

Baculovirus Surface Display of SARS Coronavirus (SARS-CoV) Spike Protein and Immunogenicity of the Displayed Protein in Mice Models

Abstract

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