@article{70bea0d553694d5ab638ec020c3e568f,
title = "Automated analysis of embryonic gene expression with cellular resolution in C. elegans",
abstract = "We describe a system that permits the automated analysis of reporter gene expression in Caenorhabditis elegans with cellular resolution continuously during embryogenesis. We demonstrate its utility by defining the expression patterns of reporters for several embryonically expressed transcription factors. The invariant cell lineage permits the automated alignment of multiple expression profiles, allowing direct comparison of the expression of different genes' reporters. We also used this system to monitor perturbations to normal development involving changes both in cell-division timing and in cell fate. Systematic application of this system could reveal the gene activity of each cell throughout development.",
author = "Murray, {John Isaac} and Zhirong Bao and Boyle, {Thomas J} and Boeck, {Max E} and Mericle, {Barbara L} and Nicholas, {Thomas J} and Zhongying Zhao and Sandel, {Matthew J} and Waterston, {Robert H}",
note = "Funding information: We thank J. Thomas, J. Priess and members of their labs for helpful discussions. We also thank J. Thomas for critical comments on the manuscript. We thank A. Audhya (University of Wisconsin, Madison) for providing worm-optimized mCherry before publication and J. Waddle (Southern Methodist University) for providing unpublished HIS-24 protein fusion vectors. Some nematode strains used in this work were provided by the Caenorhabditis Genetics Center, which is funded by the US National Institutes of Health National Center for Research Resources. This work was partially supported by funding from the National Institutes of Health. J.I.M. is a fellow of the Jane Coffin Childs Memorial Fund for Medical Research. Z.B. is a Damon Runyon Fellow supported by Damon Runyon Cancer Research Fellowship (DRG-1813-04). Publisher copyright: Copyright {\textcopyright} 2008, Nature Publishing Group",
year = "2008",
month = aug,
doi = "10.1038/nmeth.1228",
language = "English",
volume = "5",
pages = "703–709",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Publishing Group",
number = "8",
}