TY - JOUR
T1 - Autofluorescence-Free Live-Cell Imaging Using Terbium Nanoparticles
AU - Cardoso Dos Santos, M.
AU - Goetz, J.
AU - Bartenlian, H.
AU - Wong, K. L.
AU - Charbonnière, L. J.
AU - Hildebrandt, N.
N1 - Funding Information:
The authors thank the French Agence National de la Recherche (ANR project “neutrinos”) and the European Commission (Horizon2020, FET-Open project “PROSEQO”) for financial support.
PY - 2018/4/18
Y1 - 2018/4/18
N2 - Fluorescent nanoparticles (NPs) have become irreplaceable tools for advanced cellular and subcellular imaging. While very bright NPs require excitation with UV or visible light, which can create strong autofluorescence of biological components, NIR-excitable NPs without autofluorescence issues exhibit much lower brightness. Here, we show the application of a new type of surface-photosensitized terbium NPs (Tb-NPs) for autofluorescence-free intracellular imaging in live HeLa cells. The combination of exceptionally high brightness, high photostability, and long photoluminecence (PL) lifetimes for highly efficient suppression of the short-lived autofluorescence allowed for time-gated PL imaging of intracellular vesicles over 72 h without toxicity and at extremely low Tb-NP concentrations down to 12 pM. Detection of highly resolved long-lifetime (ms) PL decay curves from small (∼10 μm2) areas within single cells within a few seconds emphasized the unprecedented photophysical properties of Tb-NPs for live-cell imaging that extend well beyond currently available nanometric imaging agents.
AB - Fluorescent nanoparticles (NPs) have become irreplaceable tools for advanced cellular and subcellular imaging. While very bright NPs require excitation with UV or visible light, which can create strong autofluorescence of biological components, NIR-excitable NPs without autofluorescence issues exhibit much lower brightness. Here, we show the application of a new type of surface-photosensitized terbium NPs (Tb-NPs) for autofluorescence-free intracellular imaging in live HeLa cells. The combination of exceptionally high brightness, high photostability, and long photoluminecence (PL) lifetimes for highly efficient suppression of the short-lived autofluorescence allowed for time-gated PL imaging of intracellular vesicles over 72 h without toxicity and at extremely low Tb-NP concentrations down to 12 pM. Detection of highly resolved long-lifetime (ms) PL decay curves from small (∼10 μm2) areas within single cells within a few seconds emphasized the unprecedented photophysical properties of Tb-NPs for live-cell imaging that extend well beyond currently available nanometric imaging agents.
UR - http://www.scopus.com/inward/record.url?scp=85045559041&partnerID=8YFLogxK
U2 - 10.1021/acs.bioconjchem.8b00069
DO - 10.1021/acs.bioconjchem.8b00069
M3 - Journal article
C2 - 29461805
AN - SCOPUS:85045559041
SN - 1043-1802
VL - 29
SP - 1327
EP - 1334
JO - Bioconjugate Chemistry
JF - Bioconjugate Chemistry
IS - 4
ER -