Augmentation of Tumor Necrosis Factor-α-Induced Monocytic Differentiation of a Myelomonocytic Leukemia (WEHI-3B JCS) by Pertussis Toxin

Nai Ki MAK*, Kwok Nam Leung, Ming Chui Fung, Andrew J. Hapel

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

We have shown that pertussis toxin (PTx) modulates the effect of tumor necrosis factor-alpha (TNF-α) in inducing monocytic differentiation of WEHI-3B (JCS) myeloid leukemic cells in vitro. PTx (0.1–2 ng/ml) alone was not cytotoxic and did not induce morphological changes in JCS cells. In the presence of a suboptimal concentration of TNF-α (25 U/ml), however, PTx (1 ng/ml) acted synergistically in inhibiting proliferation and in inducing monocytic differentiation of the JCS cells. Expression of the macrophage differentiation marker (Mac-1) on JCS cells was increased by the combination of PTx and TNF-α, and phagocytic activity of the cells was also enhanced. Moreover, JCS cells treated with PTx and TNF-α had reduced tumorigenic capacity in vivo. The data suggest that a PTx-sensitive G protein may be involved in regulating the TNF-α-induced monocytic differentiation of the myeloid leukemic JCS cells and that combination of PTx and TNF-α may be useful in the treatment of some forms of myelomonocytic leukemia.

Original languageEnglish
Pages (from-to)1-12
Number of pages12
JournalImmunobiology
Volume190
Issue number1-2
DOIs
Publication statusPublished - 1994

Scopus Subject Areas

  • Immunology and Allergy
  • Immunology
  • Hematology

User-Defined Keywords

  • G protein
  • guanine-nucleotide-binding protein
  • mean survival time
  • MST
  • PBS
  • pertussis toxin
  • phosphate-buffered saline
  • PTx
  • TNF-α
  • tumor necrosis factor-alpha

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