AtDsPTP1 acts as a negative regulator in osmotic stress signalling during Arabidopsis seed germination and seedling establishment

Rui Liu, Yinggao Liu, Nenghui Ye, Guohui Zhu, Moxian Chen, Liguo Jia, Yiji XIA, Lu Shi, Wensuo Jia, Jianhua ZHANG*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

Dual-specificity protein phosphatases (DsPTPs) target both tyrosine and serine/threonine residues and play roles in plant growth and development. We have characterized an Arabidopsis mutant, dsptp1, which shows a higher seed germination rate and better root elongation under osmotic stress than the wild type. By contrast, its overexpression line, DsPTP1-OE, shows inhibited seed germination and root elongation; and its complemented line, DsPTP1-Com, resembles the wild type and rescues DsPTP1-OE under osmotic stress. Expression of AtDsPTP1 is enhanced by osmotic stress in seed coats, bases of rosette leaves, and roots. Compared with the wild type, the dsptp1 mutant shows increased proline accumulation, reduced malondialdehyde (MDA) content and ion leakage, and enhanced antioxidant enzyme activity in response to osmotic stress. AtDsPTP1 regulates the transcript levels of various dehydration-responsive genes under osmotic stress. Abscisic acid (ABA) accumulation in dsptp1 under osmotic stress is reduced with reduced expression of the ABA-biosynthesis gene NCED3 and increased expression of the ABA-catabolism gene CYP707A4. AtDsPTP1 also regulates the expression of key components in the ABA-signalling pathway. In conclusion, AtDsPTP1 regulates ABA accumulation, and acts as a negative regulator in osmotic stress signalling during Arabidospsis seed germination and seedling establishment.

Original languageEnglish
Pages (from-to)1339-1353
Number of pages15
JournalJournal of Experimental Botany
Volume66
Issue number5
DOIs
Publication statusPublished - 1 Mar 2015

Scopus Subject Areas

  • Physiology
  • Plant Science

User-Defined Keywords

  • Abscisic acid (ABA)
  • Arabidopsis
  • Dual-specificity protein phosphatase
  • Osmotic stress
  • Root elongation
  • Seed germination

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