AT14A mediates the cell wall-plasma membrane-cytoskeleton continuum in Arabidopsis thaliana cells

Bing Lü, Juan Wang, Yu Zhang, Hongcheng Wang, Jiansheng Liang, Jianhua ZHANG*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

AT14A has a small domain that has sequence similarities to integrins from animals. Integrins serve as a transmembrane linker between the extracellular matrix and the cytoskeleton, which play critical roles in a variety of biological processes. Because the function of AT14A is unknown, Arabidopsis thaliana AT14A, which is a transmembrane receptor for cell adhesion molecules and a middle member of the cell wall-plasma membrane- cytoskeleton continuum in plants, has been described. AT14A, co-expressed with green fluorescent protein (GFP), was found to localize mainly to the plasma membrane. The mutant Arabidopsis at14a-1 cells exhibit various phenotypes with cell shape, cell cluster size, thickness, and cellulose content of cell wall, the adhesion between cells, and the adhesion of plasma membrane to cell wall varied by plasmolysis. Using direct staining of filamentous actin and indirect immunofluorescence staining of microtubules, cortical actin filaments and microtubules arrays were significantly altered in cells, either where AT14A was absent or over-expressed. It is concluded that AT14A may be a substantial middle member of the cell wall-plasma membrane-cytoskeleton continuum and play an important role in the continuum by regulating cell wall and cortical cytoskeleton organization.

Original languageEnglish
Pages (from-to)4061-4069
Number of pages9
JournalJournal of Experimental Botany
Volume63
Issue number11
DOIs
Publication statusPublished - Jun 2012

Scopus Subject Areas

  • Physiology
  • Plant Science

User-Defined Keywords

  • Arabidopsis thaliana
  • AT14A
  • Cell wall-plasma membrane-cytoskeleton continuum
  • Transmembrane linker

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