Assemblies of brilliant cresyl violet to DNA in the presence of γ-cyclodextrin

Jin Ping Song; Yu Jing Guo; Qiang Zhao; Shao Min Shuang; Chuan Dong; Martin M.F. Choi

doi:10.1016/j.talanta.2010.05.032

Assemblies of brilliant cresyl violet to DNA in the presence of γ-cyclodextrin

Jin Ping Song, Yu Jing Guo, Qiang Zhao, Shao Min Shuang, Chuan Dong^*, Martin M.F. Choi

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

9 Citations (Scopus)

Abstract

The interactions of brilliant cresyl violet (BCV) with herring sperm DNA in γ-cyclodextrin (γ-CD) supramolecular system were studied by UV-vis absorption spectroscopy and cyclic voltammetry (CV). Both UV-vis absorption and CV data show that the interaction of BCV with DNA depends on the concentration ratio of BCV to DNA (R), the initial concentration of BCV and γ-CD. The binding constants of BCV monomer, (BCV)₂ dimer and (BCV) ₂-γ-CD inclusion complex with DNA are 1.64 × 10 ⁵, 2.56 × 10⁴ and 2.32 × 10³ M ^-1, respectively. It was observed that γ-CD can affect the interactive mode of BCV with DNA. If R is larger than 0.5, the (BCV) ₂-γ-CD inclusion complex will retain intact and bind to DNA via the electrostatic attraction forces. By contrast, when R is smaller than 0.5, the inclusion complex will be partially dissociated and the free BCV monomer is intercalated into the double-helix structure of DNA attributing to the more favorable microenvironment of DNA for the BCV monomer. Our work postulates the importance of the initial concentration of dye and host molecule on the interaction of dye with DNA in living bodies.

Original language	English
Pages (from-to)	681-686
Number of pages	6
Journal	Talanta
Volume	82
Issue number	2
DOIs	https://doi.org/10.1016/j.talanta.2010.05.032
Publication status	Published - 15 Jul 2010

Scopus Subject Areas

Analytical Chemistry

User-Defined Keywords

Brilliant cresyl violet
DNA
Inclusion complex
γ-Cyclodextrin

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10.1016/j.talanta.2010.05.032

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title = "Assemblies of brilliant cresyl violet to DNA in the presence of γ-cyclodextrin",

abstract = "The interactions of brilliant cresyl violet (BCV) with herring sperm DNA in γ-cyclodextrin (γ-CD) supramolecular system were studied by UV-vis absorption spectroscopy and cyclic voltammetry (CV). Both UV-vis absorption and CV data show that the interaction of BCV with DNA depends on the concentration ratio of BCV to DNA (R), the initial concentration of BCV and γ-CD. The binding constants of BCV monomer, (BCV)2 dimer and (BCV) 2-γ-CD inclusion complex with DNA are 1.64 × 10 5, 2.56 × 104 and 2.32 × 103 M -1, respectively. It was observed that γ-CD can affect the interactive mode of BCV with DNA. If R is larger than 0.5, the (BCV) 2-γ-CD inclusion complex will retain intact and bind to DNA via the electrostatic attraction forces. By contrast, when R is smaller than 0.5, the inclusion complex will be partially dissociated and the free BCV monomer is intercalated into the double-helix structure of DNA attributing to the more favorable microenvironment of DNA for the BCV monomer. Our work postulates the importance of the initial concentration of dye and host molecule on the interaction of dye with DNA in living bodies.",

keywords = "Brilliant cresyl violet, DNA, Inclusion complex, γ-Cyclodextrin",

author = "Song, {Jin Ping} and Guo, {Yu Jing} and Qiang Zhao and Shuang, {Shao Min} and Chuan Dong and Choi, {Martin M.F.}",

note = "Funding Information: This work was supported by the National Natural Science Foundation of China (20875059). The authors express their sincere thanks to the reviewers for their valuable suggestions to improve the quality of this manuscript. ",

year = "2010",

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doi = "10.1016/j.talanta.2010.05.032",

language = "English",

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T1 - Assemblies of brilliant cresyl violet to DNA in the presence of γ-cyclodextrin

AU - Song, Jin Ping

AU - Guo, Yu Jing

AU - Zhao, Qiang

AU - Shuang, Shao Min

AU - Dong, Chuan

AU - Choi, Martin M.F.

N1 - Funding Information: This work was supported by the National Natural Science Foundation of China (20875059). The authors express their sincere thanks to the reviewers for their valuable suggestions to improve the quality of this manuscript.

PY - 2010/7/15

Y1 - 2010/7/15

N2 - The interactions of brilliant cresyl violet (BCV) with herring sperm DNA in γ-cyclodextrin (γ-CD) supramolecular system were studied by UV-vis absorption spectroscopy and cyclic voltammetry (CV). Both UV-vis absorption and CV data show that the interaction of BCV with DNA depends on the concentration ratio of BCV to DNA (R), the initial concentration of BCV and γ-CD. The binding constants of BCV monomer, (BCV)2 dimer and (BCV) 2-γ-CD inclusion complex with DNA are 1.64 × 10 5, 2.56 × 104 and 2.32 × 103 M -1, respectively. It was observed that γ-CD can affect the interactive mode of BCV with DNA. If R is larger than 0.5, the (BCV) 2-γ-CD inclusion complex will retain intact and bind to DNA via the electrostatic attraction forces. By contrast, when R is smaller than 0.5, the inclusion complex will be partially dissociated and the free BCV monomer is intercalated into the double-helix structure of DNA attributing to the more favorable microenvironment of DNA for the BCV monomer. Our work postulates the importance of the initial concentration of dye and host molecule on the interaction of dye with DNA in living bodies.

AB - The interactions of brilliant cresyl violet (BCV) with herring sperm DNA in γ-cyclodextrin (γ-CD) supramolecular system were studied by UV-vis absorption spectroscopy and cyclic voltammetry (CV). Both UV-vis absorption and CV data show that the interaction of BCV with DNA depends on the concentration ratio of BCV to DNA (R), the initial concentration of BCV and γ-CD. The binding constants of BCV monomer, (BCV)2 dimer and (BCV) 2-γ-CD inclusion complex with DNA are 1.64 × 10 5, 2.56 × 104 and 2.32 × 103 M -1, respectively. It was observed that γ-CD can affect the interactive mode of BCV with DNA. If R is larger than 0.5, the (BCV) 2-γ-CD inclusion complex will retain intact and bind to DNA via the electrostatic attraction forces. By contrast, when R is smaller than 0.5, the inclusion complex will be partially dissociated and the free BCV monomer is intercalated into the double-helix structure of DNA attributing to the more favorable microenvironment of DNA for the BCV monomer. Our work postulates the importance of the initial concentration of dye and host molecule on the interaction of dye with DNA in living bodies.

KW - Brilliant cresyl violet

KW - DNA

KW - Inclusion complex

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Assemblies of brilliant cresyl violet to DNA in the presence of γ-cyclodextrin

Abstract

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