Arabidopsis PUB2 and PUB4 connect signaling components of pattern-triggered immunity

Yiping Wang*, Yingying Wu, Huan Zhong, Shuai Chen, Kam Bo Wong, Yiji Xia*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Plants use pattern recognition receptors (PRRs) to detect pathogen-associated molecular patterns (PAMPs) and activate pattern-triggered immunity (PTI). Precise regulation of information from PRRs to downstream signaling components is vital to mounting an appropriate immune response and requires dynamic interactions of these PTI components. We used transcriptome profiling, phenotypic analysis, molecular genetics, and protein–protein interaction analysis to understand the roles of the Arabidopsis plant U-box (PUB) proteins PUB2 and PUB4 in disease resistance and PTI signaling. Loss of function of both PUB2 and PUB4 diminishes the PAMP-triggered oxidative bursts and dampens mitogen-activated protein kinase signaling, resulting in a severe compromise in resistance to not only pathogenic but also nonpathogenic strains of Pseudomonas syringae. Within PUB4, the E3 ligase activity is dispensable, but the armadillo repeat region is essential and sufficient for its function in immunity. PUB2 and PUB4 interact with PTI signaling components, including FLS2, BIK1, PBL27, and RbohD, and enhance FLS2-BIK1 and BIK1-RbohD interactions. Our study reveals that PUB2 and PUB4 are critical components of plant immunity and connect PTI components to positively regulate defense responses.

Original languageEnglish
Pages (from-to)2249-2265
Number of pages17
JournalNew Phytologist
Volume233
Issue number5
Early online date17 Dec 2021
DOIs
Publication statusPublished - Mar 2022

Scopus Subject Areas

  • Physiology
  • Plant Science

User-Defined Keywords

  • ARM repeat
  • Arabidopsis
  • E3 ligase
  • PUB2
  • PUB4
  • pattern-triggered immunity

Fingerprint

Dive into the research topics of 'Arabidopsis PUB2 and PUB4 connect signaling components of pattern-triggered immunity'. Together they form a unique fingerprint.

Cite this