TY - JOUR
T1 - Arabidopsis DXO1 activates RNMT1 to methylate the mRNA guanosine cap
AU - Xiao, Chen
AU - Li, Kaien
AU - Hua, Jingmin
AU - He, Zhao
AU - Zhang, Feng
AU - Li, Qiongfang
AU - Zhang, Hailei
AU - Yang, Lei
AU - Pan, Shuying
AU - Cai, Zongwei
AU - Yu, Zhiling
AU - Wong, Kam Bo
AU - Xia, Yiji
N1 - Funding Information:
We thank Ms. Anita K. Snyder for English editing. This work was supported by the Research Grants Council of Hong Kong (GRF grant nos. 12100018, 12102220, and 12101721 to Y.X., C2009-19GF to Z.C., K.W., and Y.X., and AoE/M-403/16 to K.W. and Y.X.) and Hong Kong Baptist University (RC-ICRS/16-17/04 to Y.X. and Z.C.
Publisher Copyright:
© 2023, The Author(s).
PY - 2023/1/13
Y1 - 2023/1/13
N2 - Eukaryotic messenger RNA (mRNA) typically contains a methylated guanosine (m7G) cap, which mediates major steps of mRNA metabolism. Recently, some RNAs in both prokaryotic and eukaryotic organisms have been found to carry a non-canonical cap such as the NAD cap. Here we report that Arabidopsis DXO family protein AtDXO1, which was previously known to be a decapping enzyme for NAD-capped RNAs (NAD-RNA), is an essential component for m7G capping. AtDXO1 associates with and activates RNA guanosine-7 methyltransferase (AtRNMT1) to catalyze conversion of the guanosine cap to the m7G cap. AtRNMT1 is an essential gene. Partial loss-of-function mutations of AtRNMT1 and knockout mutation of AtDXO1 reduce m7G-capped mRNA but increase G-capped mRNAs, leading to similar pleiotropic phenotypes, whereas overexpression of AtRNMT1 partially restores the atdxo1 phenotypes. This work reveals an important mechanism in m7G capping in plants by which the NAD-RNA decapping enzyme AtDXO1 is required for efficient guanosine cap methylation.
AB - Eukaryotic messenger RNA (mRNA) typically contains a methylated guanosine (m7G) cap, which mediates major steps of mRNA metabolism. Recently, some RNAs in both prokaryotic and eukaryotic organisms have been found to carry a non-canonical cap such as the NAD cap. Here we report that Arabidopsis DXO family protein AtDXO1, which was previously known to be a decapping enzyme for NAD-capped RNAs (NAD-RNA), is an essential component for m7G capping. AtDXO1 associates with and activates RNA guanosine-7 methyltransferase (AtRNMT1) to catalyze conversion of the guanosine cap to the m7G cap. AtRNMT1 is an essential gene. Partial loss-of-function mutations of AtRNMT1 and knockout mutation of AtDXO1 reduce m7G-capped mRNA but increase G-capped mRNAs, leading to similar pleiotropic phenotypes, whereas overexpression of AtRNMT1 partially restores the atdxo1 phenotypes. This work reveals an important mechanism in m7G capping in plants by which the NAD-RNA decapping enzyme AtDXO1 is required for efficient guanosine cap methylation.
UR - http://www.scopus.com/inward/record.url?scp=85146290765&partnerID=8YFLogxK
U2 - 10.1038/s41467-023-35903-8
DO - 10.1038/s41467-023-35903-8
M3 - Journal article
C2 - 36639378
AN - SCOPUS:85146290765
SN - 2041-1723
VL - 14
JO - Nature Communications
JF - Nature Communications
M1 - 202
ER -
