Application of rumen microbes to enhance food waste hydrolysis in acidogenic leach-bed reactors

Bing Hua Yan; Ammaiyappan Selvam; Jonathan W.C. Wong

doi:10.1016/j.biortech.2014.03.085

Application of rumen microbes to enhance food waste hydrolysis in acidogenic leach-bed reactors

Bing Hua Yan, Ammaiyappan Selvam, Jonathan W.C. Wong^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

42 Citations (Scopus)

Abstract

Effect of rumen microorganisms on hydrolysis of food waste in leach bed reactor (LBR) was investigated. LBRs were inoculated (20%, w/w) with cow manure and anaerobically digested sludge at different ratios, 0:1 (LBR-A), 1:3 (LBR-B), 1:1 (LBR-C), 3:1 (LBR-D) and 1:0 (LBR-E). High volatile solids (VS) conversion efficiency of 68% was achieved in LBR-E. Compared with LBR-A, chemical oxygen demand, total soluble products and total Kjeldahl nitrogen leaching of LBR-E were increased by 16%, 14.3% and 27%, respectively. Recovery of the highest amounts of ethanol and butyrate in LBR-E indicated that the metabolic pathway mediated by rumen microorganisms was favorable for subsequent methanogenesis. Phylogenetic analysis confirmed that the enhanced hydrolysis in LBR-E was mainly due to strong degraders, e.g. Enterobacter, Bifidobacterium thermacidophilum and Caloramator sourced from cow manure. Results demonstrate that rumen microorganisms rapidly degrade the VS and produce useful VFAs with high methane yields in subsequent methanogenesis.

Original language	English
Pages (from-to)	64-71
Number of pages	8
Journal	Bioresource Technology
Volume	168
Early online date	25 Mar 2014
DOIs	https://doi.org/10.1016/j.biortech.2014.03.085
Publication status	Published - Sept 2014

Scopus Subject Areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

User-Defined Keywords

Anaerobic hydrolysis
Food waste
Leach bed reactor
Rumen microbes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2014.03.085

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title = "Application of rumen microbes to enhance food waste hydrolysis in acidogenic leach-bed reactors",

abstract = "Effect of rumen microorganisms on hydrolysis of food waste in leach bed reactor (LBR) was investigated. LBRs were inoculated (20%, w/w) with cow manure and anaerobically digested sludge at different ratios, 0:1 (LBR-A), 1:3 (LBR-B), 1:1 (LBR-C), 3:1 (LBR-D) and 1:0 (LBR-E). High volatile solids (VS) conversion efficiency of 68% was achieved in LBR-E. Compared with LBR-A, chemical oxygen demand, total soluble products and total Kjeldahl nitrogen leaching of LBR-E were increased by 16%, 14.3% and 27%, respectively. Recovery of the highest amounts of ethanol and butyrate in LBR-E indicated that the metabolic pathway mediated by rumen microorganisms was favorable for subsequent methanogenesis. Phylogenetic analysis confirmed that the enhanced hydrolysis in LBR-E was mainly due to strong degraders, e.g. Enterobacter, Bifidobacterium thermacidophilum and Caloramator sourced from cow manure. Results demonstrate that rumen microorganisms rapidly degrade the VS and produce useful VFAs with high methane yields in subsequent methanogenesis.",

keywords = "Anaerobic hydrolysis, Food waste, Leach bed reactor, Rumen microbes",

author = "Yan, {Bing Hua} and Ammaiyappan Selvam and Wong, {Jonathan W.C.}",

note = "Funding Information: The authors thank the Research Grant Council of the Hong Kong Special Administrative Region (Grant HKBU262013) for the financial support. Publisher copyright: Copyright {\textcopyright} 2014 Elsevier Ltd. All rights reserved.",

year = "2014",

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doi = "10.1016/j.biortech.2014.03.085",

language = "English",

volume = "168",

pages = "64--71",

journal = "Bioresource Technology",

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T1 - Application of rumen microbes to enhance food waste hydrolysis in acidogenic leach-bed reactors

AU - Yan, Bing Hua

AU - Selvam, Ammaiyappan

AU - Wong, Jonathan W.C.

N1 - Funding Information: The authors thank the Research Grant Council of the Hong Kong Special Administrative Region (Grant HKBU262013) for the financial support. Publisher copyright: Copyright © 2014 Elsevier Ltd. All rights reserved.

PY - 2014/9

Y1 - 2014/9

N2 - Effect of rumen microorganisms on hydrolysis of food waste in leach bed reactor (LBR) was investigated. LBRs were inoculated (20%, w/w) with cow manure and anaerobically digested sludge at different ratios, 0:1 (LBR-A), 1:3 (LBR-B), 1:1 (LBR-C), 3:1 (LBR-D) and 1:0 (LBR-E). High volatile solids (VS) conversion efficiency of 68% was achieved in LBR-E. Compared with LBR-A, chemical oxygen demand, total soluble products and total Kjeldahl nitrogen leaching of LBR-E were increased by 16%, 14.3% and 27%, respectively. Recovery of the highest amounts of ethanol and butyrate in LBR-E indicated that the metabolic pathway mediated by rumen microorganisms was favorable for subsequent methanogenesis. Phylogenetic analysis confirmed that the enhanced hydrolysis in LBR-E was mainly due to strong degraders, e.g. Enterobacter, Bifidobacterium thermacidophilum and Caloramator sourced from cow manure. Results demonstrate that rumen microorganisms rapidly degrade the VS and produce useful VFAs with high methane yields in subsequent methanogenesis.

AB - Effect of rumen microorganisms on hydrolysis of food waste in leach bed reactor (LBR) was investigated. LBRs were inoculated (20%, w/w) with cow manure and anaerobically digested sludge at different ratios, 0:1 (LBR-A), 1:3 (LBR-B), 1:1 (LBR-C), 3:1 (LBR-D) and 1:0 (LBR-E). High volatile solids (VS) conversion efficiency of 68% was achieved in LBR-E. Compared with LBR-A, chemical oxygen demand, total soluble products and total Kjeldahl nitrogen leaching of LBR-E were increased by 16%, 14.3% and 27%, respectively. Recovery of the highest amounts of ethanol and butyrate in LBR-E indicated that the metabolic pathway mediated by rumen microorganisms was favorable for subsequent methanogenesis. Phylogenetic analysis confirmed that the enhanced hydrolysis in LBR-E was mainly due to strong degraders, e.g. Enterobacter, Bifidobacterium thermacidophilum and Caloramator sourced from cow manure. Results demonstrate that rumen microorganisms rapidly degrade the VS and produce useful VFAs with high methane yields in subsequent methanogenesis.

KW - Anaerobic hydrolysis

KW - Food waste

KW - Leach bed reactor

KW - Rumen microbes

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Application of rumen microbes to enhance food waste hydrolysis in acidogenic leach-bed reactors

Abstract

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User-Defined Keywords

UN SDGs

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