TY - JOUR
T1 - Application of rumen microbes to enhance food waste hydrolysis in acidogenic leach-bed reactors
AU - Yan, Bing Hua
AU - Selvam, Ammaiyappan
AU - Wong, Jonathan W.C.
N1 - Funding Information:
The authors thank the Research Grant Council of the Hong Kong Special Administrative Region (Grant HKBU262013) for the financial support.
Publisher copyright:
Copyright © 2014 Elsevier Ltd. All rights reserved.
PY - 2014/9
Y1 - 2014/9
N2 - Effect of rumen microorganisms on hydrolysis of food waste in leach bed
reactor (LBR) was investigated. LBRs were inoculated (20%, w/w) with cow
manure and anaerobically digested sludge at different ratios, 0:1
(LBR-A), 1:3 (LBR-B), 1:1 (LBR-C), 3:1 (LBR-D) and 1:0 (LBR-E). High
volatile solids (VS) conversion efficiency of 68% was achieved in LBR-E.
Compared with LBR-A, chemical oxygen demand, total soluble products and
total Kjeldahl nitrogen leaching of LBR-E were increased by 16%, 14.3%
and 27%, respectively. Recovery of the highest amounts of ethanol and
butyrate in LBR-E indicated that the metabolic pathway mediated by rumen
microorganisms was favorable for subsequent methanogenesis.
Phylogenetic analysis confirmed that the enhanced hydrolysis in LBR-E
was mainly due to strong degraders, e.g. Enterobacter, Bifidobacterium thermacidophilum and Caloramator
sourced from cow manure. Results demonstrate that rumen microorganisms
rapidly degrade the VS and produce useful VFAs with high methane yields
in subsequent methanogenesis.
AB - Effect of rumen microorganisms on hydrolysis of food waste in leach bed
reactor (LBR) was investigated. LBRs were inoculated (20%, w/w) with cow
manure and anaerobically digested sludge at different ratios, 0:1
(LBR-A), 1:3 (LBR-B), 1:1 (LBR-C), 3:1 (LBR-D) and 1:0 (LBR-E). High
volatile solids (VS) conversion efficiency of 68% was achieved in LBR-E.
Compared with LBR-A, chemical oxygen demand, total soluble products and
total Kjeldahl nitrogen leaching of LBR-E were increased by 16%, 14.3%
and 27%, respectively. Recovery of the highest amounts of ethanol and
butyrate in LBR-E indicated that the metabolic pathway mediated by rumen
microorganisms was favorable for subsequent methanogenesis.
Phylogenetic analysis confirmed that the enhanced hydrolysis in LBR-E
was mainly due to strong degraders, e.g. Enterobacter, Bifidobacterium thermacidophilum and Caloramator
sourced from cow manure. Results demonstrate that rumen microorganisms
rapidly degrade the VS and produce useful VFAs with high methane yields
in subsequent methanogenesis.
KW - Anaerobic hydrolysis
KW - Food waste
KW - Leach bed reactor
KW - Rumen microbes
UR - http://www.scopus.com/inward/record.url?scp=84905025885&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2014.03.085
DO - 10.1016/j.biortech.2014.03.085
M3 - Journal article
C2 - 24785786
AN - SCOPUS:84905025885
SN - 0960-8524
VL - 168
SP - 64
EP - 71
JO - Bioresource Technology
JF - Bioresource Technology
ER -
