Application of recombinant Pediococcus acidilactici BD16 (fcs +/ech +) for bioconversion of agrowaste to vanillin

Debkumar Chakraborty, Ammaiyappan Selvam, Baljinder Kaur*, Jonathan W C WONG, Obulisamy Parthiba Karthikeyan

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

32 Citations (Scopus)


Biotechnological production of vanillin is gaining momentum as the natural synthesis of vanillin that is very expensive. Ferulic acid (FA), a costly compound, is used as the substrate to produce vanillin biotechnologically and the making process is still expensive. Therefore, this study investigated the practical use of an agrobiomass waste, rice bran, and provides the first evidence of a cost-effective production of vanillin within 24 h of incubation using recombinant Pediococcus acidilactici BD16 (fcs+/ech+). Introduction of two genes encoding feruloyl CoA synthetase and enoyl CoA hydratase into the native strain increased vanillin yield to 4.01 g L−1. Bioconversion was monitored through the transformation of phenolic compounds. A hypothetical metabolic pathway of rice bran during the vanillin bioconversion was proposed with the inserted pathway from ferulic acid to vanillin and compared with that of other metabolic engineered strains. These results could be a gateway of using recombinant lactic acid bacteria for industrial production of vanillin from agricultural waste.

Original languageEnglish
Pages (from-to)5615-5626
Number of pages12
JournalApplied Microbiology and Biotechnology
Issue number14
Publication statusPublished - 1 Jul 2017

Scopus Subject Areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

User-Defined Keywords

  • Agro waste
  • Phenolic metabolites
  • Recombinant Pediococcus acidilactici
  • Rice bran
  • Vanillin


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