Application of microscopy technique and high performance liquid chromatography for quality assessment of Polygonum multiflorum Thunb. (Heshouwu)

Li Liang, Zhongzhen ZHAO, Tingguo Kang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Background: The technique of microscopy has been applied for identification of Chinese materia medica (CMM) since decades. However, very few scientific publications report the combination of conventional microscopy and high performance liquid chromatography (HPLC) techniques for further application to quality assessment of CMM.

Objective: The objective of this study is to analyze the quality of the dried root tuber of Polygonum multiflorum Thunb. (Heshouwu) and to establish the relationships between 2,3,5,4′- tetrahydroxystilbene-2-O-β-glucoside, combined anthraquinone (CAQ) and quantity of clusters of calcium oxalate.

Materials and Methods: In this study, microscopy and HPLC techniques were applied to assess the quality of P. multiflorum Thunb., and SPSS software was used to establish the relationship between microscopic characteristics and chemical components.

Results: The results showed close and direct correlations between the quantity of clusters of calcium oxalate in P. multiflorum Thunb. and the contents of 2,3,5,4′- tetrahydroxystilbene-2-O-β-glucoside and CAQ. From these results, it can be deduced that Polygoni Multiflori Radix with a higher quantity of clusters of calcium oxalate should be of better quality.

Conclusion: The established method can be helpful for evaluating the quality of CMM based upon the identification and quantitation of chemical and ergastic substance of cells.

Original languageEnglish
Pages (from-to)415-421
Number of pages7
JournalPharmacognosy Magazine
Volume10
Issue number40
DOIs
Publication statusPublished - 1 Oct 2014

Scopus Subject Areas

  • Pharmaceutical Science
  • Drug Discovery

User-Defined Keywords

  • 2,3,5,4′- tetrahydroxystilbene-2-O-β-glucoside
  • clusters of calcium oxalates
  • combined anthraquinone
  • Polygonum multiflorum Thunb
  • quantitative analysis

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