Application of hydrophobic palladium nanoparticles for the development of electrochemical glucose biosensor

Zhongping Li; Xu Wang; Guangming Wen; Shaomin Shuang; Chuan Dong; Man Chin Paau; Martin M.F. Choi

doi:10.1016/j.bios.2011.04.057

Application of hydrophobic palladium nanoparticles for the development of electrochemical glucose biosensor

Zhongping Li, Xu Wang, Guangming Wen, Shaomin Shuang, Chuan Dong^*, Man Chin Paau, Martin M.F. Choi

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

26 Citations (Scopus)

Abstract

An amperometric glucose biosensor based on an n-alkylamine-stabilized palladium nanoparticles (PdNPs)-glucose oxidase (GOx) modified glassy carbon (GC) electrode has been successfully fabricated. PdNPs were initially synthesized by a biphase mixture of water and toluene method using n-alkylamines (dodecylamine, C ₁₂-NH ₂ and octadecylamine, C ₁₈-NH ₂) as stabilizing ligands. The performance of the PdNPs-GOx/GC biosensor was studied by cyclic voltammetry. The optimum working potential for amperometric measurement of glucose in pH 7.0 phosphate buffer solution is -0.02V (vs. Ag/AgCl). The analytical performance of the biosensor prepared from C ₁₈-PdNPs-GOx is better than that of C ₁₂-PdNPs-GOx. The C ₁₈-PdNPs-GOx/GC biosensor exhibits a fast response time of ca. 3s, a detection limit of 3.0μM (S/N=3) and a linear range of 3.0μM-8.0mM. The linear dependence of current density with glucose concentration is 70.8μAcm ^-2mM ^-1. The biosensor shows good stability, repeatability and reproducibility. It has been successfully applied to determine the glucose content in human blood serum samples.

Original language	English
Pages (from-to)	4619-4623
Number of pages	5
Journal	Biosensors and Bioelectronics
Volume	26
Issue number	11
DOIs	https://doi.org/10.1016/j.bios.2011.04.057
Publication status	Published - 15 Jul 2011

Scopus Subject Areas

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

User-Defined Keywords

Amperometry
Glucose
Palladium nanoparticles

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10.1016/j.bios.2011.04.057

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title = "Application of hydrophobic palladium nanoparticles for the development of electrochemical glucose biosensor",

abstract = "An amperometric glucose biosensor based on an n-alkylamine-stabilized palladium nanoparticles (PdNPs)-glucose oxidase (GOx) modified glassy carbon (GC) electrode has been successfully fabricated. PdNPs were initially synthesized by a biphase mixture of water and toluene method using n-alkylamines (dodecylamine, C 12-NH 2 and octadecylamine, C 18-NH 2) as stabilizing ligands. The performance of the PdNPs-GOx/GC biosensor was studied by cyclic voltammetry. The optimum working potential for amperometric measurement of glucose in pH 7.0 phosphate buffer solution is -0.02V (vs. Ag/AgCl). The analytical performance of the biosensor prepared from C 18-PdNPs-GOx is better than that of C 12-PdNPs-GOx. The C 18-PdNPs-GOx/GC biosensor exhibits a fast response time of ca. 3s, a detection limit of 3.0μM (S/N=3) and a linear range of 3.0μM-8.0mM. The linear dependence of current density with glucose concentration is 70.8μAcm -2mM -1. The biosensor shows good stability, repeatability and reproducibility. It has been successfully applied to determine the glucose content in human blood serum samples.",

keywords = "Amperometry, Glucose, Palladium nanoparticles",

author = "Zhongping Li and Xu Wang and Guangming Wen and Shaomin Shuang and Chuan Dong and Paau, {Man Chin} and Choi, {Martin M.F.}",

note = "Funding Information: We express our sincere thanks to Mr. Guojin Li (China Research Institute of Daily Chemical Industry) for taking the TEM images. The work described in this article was supported by grants from the National Natural Science Foundation of China (20875059) and Science Foundation of Shanxi Province ( 2010021014 ). Financial support from the Research Grants Council of the Hong Kong Special Administrative Region , China (project no. HKBU 200907) is also gratefully acknowledged. ",

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T1 - Application of hydrophobic palladium nanoparticles for the development of electrochemical glucose biosensor

AU - Li, Zhongping

AU - Wang, Xu

AU - Wen, Guangming

AU - Shuang, Shaomin

AU - Dong, Chuan

AU - Paau, Man Chin

AU - Choi, Martin M.F.

N1 - Funding Information: We express our sincere thanks to Mr. Guojin Li (China Research Institute of Daily Chemical Industry) for taking the TEM images. The work described in this article was supported by grants from the National Natural Science Foundation of China (20875059) and Science Foundation of Shanxi Province ( 2010021014 ). Financial support from the Research Grants Council of the Hong Kong Special Administrative Region , China (project no. HKBU 200907) is also gratefully acknowledged.

PY - 2011/7/15

Y1 - 2011/7/15

N2 - An amperometric glucose biosensor based on an n-alkylamine-stabilized palladium nanoparticles (PdNPs)-glucose oxidase (GOx) modified glassy carbon (GC) electrode has been successfully fabricated. PdNPs were initially synthesized by a biphase mixture of water and toluene method using n-alkylamines (dodecylamine, C 12-NH 2 and octadecylamine, C 18-NH 2) as stabilizing ligands. The performance of the PdNPs-GOx/GC biosensor was studied by cyclic voltammetry. The optimum working potential for amperometric measurement of glucose in pH 7.0 phosphate buffer solution is -0.02V (vs. Ag/AgCl). The analytical performance of the biosensor prepared from C 18-PdNPs-GOx is better than that of C 12-PdNPs-GOx. The C 18-PdNPs-GOx/GC biosensor exhibits a fast response time of ca. 3s, a detection limit of 3.0μM (S/N=3) and a linear range of 3.0μM-8.0mM. The linear dependence of current density with glucose concentration is 70.8μAcm -2mM -1. The biosensor shows good stability, repeatability and reproducibility. It has been successfully applied to determine the glucose content in human blood serum samples.

AB - An amperometric glucose biosensor based on an n-alkylamine-stabilized palladium nanoparticles (PdNPs)-glucose oxidase (GOx) modified glassy carbon (GC) electrode has been successfully fabricated. PdNPs were initially synthesized by a biphase mixture of water and toluene method using n-alkylamines (dodecylamine, C 12-NH 2 and octadecylamine, C 18-NH 2) as stabilizing ligands. The performance of the PdNPs-GOx/GC biosensor was studied by cyclic voltammetry. The optimum working potential for amperometric measurement of glucose in pH 7.0 phosphate buffer solution is -0.02V (vs. Ag/AgCl). The analytical performance of the biosensor prepared from C 18-PdNPs-GOx is better than that of C 12-PdNPs-GOx. The C 18-PdNPs-GOx/GC biosensor exhibits a fast response time of ca. 3s, a detection limit of 3.0μM (S/N=3) and a linear range of 3.0μM-8.0mM. The linear dependence of current density with glucose concentration is 70.8μAcm -2mM -1. The biosensor shows good stability, repeatability and reproducibility. It has been successfully applied to determine the glucose content in human blood serum samples.

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ER -

Application of hydrophobic palladium nanoparticles for the development of electrochemical glucose biosensor

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