Application of Derivatization in Fatty Acids and Fatty Acyls Detection: Mass Spectrometry-Based Targeted Lipidomics

Li Xiang, Lin Zhu, Yu Huang, Zongwei CAI*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

4 Citations (Scopus)

Abstract

Mass spectrometry (MS) has become an indispensable technique in the analysis of fatty acids (FAs) and fatty acyls due to its superior sensitivity and selectivity. Chemical derivatization is often introduced in MS-based analysis because of the inherent poor ionization efficiency and selectivity of FAs and fatty acyls. This critical review presents an overview of commonly used MS platforms including gas chromatography-MS, liquid chromatography-MS, and mass spectrometry imaging combined with representative derivatization reagents for FAs and fatty acyls based on different chain-lengths, saturation degrees, and conjugation groups. The method applications and limitations as well as critical derivatization conditions in different biological matrices are summarized. Special remarks on the chemical derivatization for MS-based analysis are provided. Future perspectives associated with the detection of FAs and fatty acyls are discussed. This review provides valuable information and suggestions for the targeted detection of interested FAs and fatty acyls using different MS-based platforms.

Original languageEnglish
Article number2000160
JournalSmall Methods
Volume4
Issue number8
DOIs
Publication statusPublished - 1 Aug 2020

Scopus Subject Areas

  • Materials Science(all)
  • Chemistry(all)

User-Defined Keywords

  • derivatization
  • fatty acids
  • fatty acyls
  • GC-MS
  • LC-MS
  • mass spectrometry imaging

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