Application of capillary zone electrophoresis for separation of water-soluble gold monolayer-protected clusters

Chung Keung Lo, Man Chin Paau, Dan Xiao, Martin M F CHOI*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

An effective capillary electrophoretic technique for separating samples of negatively charged, polydisperse, water-soluble gold monolayer-protected duster (Au MPC) protected by monolayers of N-acetyl-L-cysteine has been developed. The separation mechanisms of the Au MPC in CZE suggest that the Luger core sizes Au MPC emerge first from the capillary. The electrophoretic separation depends on pH, buffer concentration, and organic modifiers. The addition of aliphatic alcohols to the run buffer can improve the separation of Au MPC by reducing the EOF and changing the selectivity between the Au MPCs. The enhancement of resolution is attributed to the more significant difference in the charge-to-size ratio between the Au MPCs. The run buffer containing 20 v/ v % ethanol provides the best separation for water-soluble Au MPC. Our proposed CE method provides a powerful tool to evaluate and separate the water-soluble Au MPC products.

Original languageEnglish
Pages (from-to)2330-2339
Number of pages10
JournalElectrophoresis
Volume29
Issue number11
DOIs
Publication statusPublished - Jun 2008

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

User-Defined Keywords

  • Capillary zone electrophoresis
  • Monolayer-protected gold clusters
  • Organic modifier

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