Application of atmospheric pressure chemical ionisation mass spectrometry in the identification and differentiation of Panax species

Kelvin S Y LEUNG*, Kelvin Chan, Alan Bensoussan, Mary J. Munroe

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

An HPLC-MS method using an atmospheric pressure chemical ionisation (APCI) source has been developed to assist in the differentiation of three ginseng species: Panax quinquefolium (American ginseng), P. ginseng (Chinese ginseng) and P. notoginseng (sanqi) species. The differentiation method relies on the identification of ginsenosides Rf and F11, and notoginsenoside R1. R1 is observed in both P. notoginseng and Chinese ginseng, whilst F11 is found exclusively in the American species. The presence of these compounds permits the definitive identification of the species to be made. The APCI ionisation source has been employed to tackle the matrix interference in analysing Chinese medicinal materials and to minimise the associated matrix effects that are commonly encountered with other ionisation modes. Moreover, the method allows direct interface to conventional HPLC systems. More importantly, chemical reference standards of ginsenosides are not required in this method. This technique provides an alternative approach to analysing high molecular weight polar compounds that typically encountered in complex matrices of Chinese medicinal materials.

Original languageEnglish
Pages (from-to)146-150
Number of pages5
JournalPhytochemical Analysis
Volume18
Issue number2
DOIs
Publication statusPublished - Mar 2007

Scopus Subject Areas

  • Analytical Chemistry
  • Food Science
  • Biochemistry
  • Molecular Medicine
  • Plant Science
  • Drug Discovery
  • Complementary and alternative medicine

User-Defined Keywords

  • Atmospheric pressure chemical ionisation
  • Ginseng
  • HPLC-MS
  • Notoginseng

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