Application of a biosensor for monitoring of ethanol

Guangming Wen, Yan Zhang, Shaomin Shuang*, Chuan Dong, Martin M F CHOI

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

64 Citations (Scopus)


An alcohol biosensor for the measurement of ethanol has been developed. It comprises an alcohol oxidase/chitosan immobilized eggshell membrane and a commercial oxygen sensor. Ethanol determination is based on the depletion of dissolved oxygen content upon exposure to ethanol solution. The decrease in oxygen level was monitored and related to the ethanol concentration. The biosensor response depends linearly on ethanol concentration between 60 μM and 0.80 mM with a detection limit of 30 μM (S/N = 3) and 1 min response time. In the optimization studies of the enzyme biosensor the most suitable enzyme and chitosan amounts were found to be 1.0 mg and 0.30% (w/v), respectively. The phosphate buffer (pH 7.4, 25 mM) and room temperature (20-25 °C) were chosen as the optimum working conditions. In the characterization studies of the ethanol biosensor some parameters such as interference effects, operational and storage stability were studied in detail. The biosensor was also tested with various wine samples. The results of this newly developed biosensor were comparable to the results obtained by a gas chromatographic method.

Original languageEnglish
Pages (from-to)121-129
Number of pages9
JournalBiosensors and Bioelectronics
Issue number1
Publication statusPublished - 30 Aug 2007

Scopus Subject Areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

User-Defined Keywords

  • Alcohol oxidase
  • Chitosan
  • Eggshell membrane
  • Ethanol


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