Apoptosis sensitization by euphorbia factor L1 in ABCB1-mediated multidrug resistant K562/ADR cells

Jian Ye Zhang; Min Ting Lin; Tao YI; Yi Na Tang; Lan Lan Fan; Xi Cheng He; Zhongzhen ZHAO; Hubiao CHEN

doi:10.3390/molecules181012793

Apoptosis sensitization by euphorbia factor L1 in ABCB1-mediated multidrug resistant K562/ADR cells

Jian Ye Zhang, Min Ting Lin, Tao YI, Yi Na Tang, Lan Lan Fan, Xi Cheng He, Zhongzhen ZHAO, Hubiao CHEN^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

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Abstract

In this article, reversal activities of Euphorbia factor L1 (EFL1) against ABCB1-mediated multidrug resistance (MDR) and apoptosis sensitization in K562/ADR cells are reported. EFL1 decreased the IC₅₀ values of anticancer agents in K562/ADR cells over-expressing ABCB1. However, EFL1 did not affect the IC₅₀ values of anticancer agents in sensitive K562 cells. Additionally, EFL1 increased the intracellular accumulation of rhodamine 123 and doxorubicin in K562/ADR cells without affecting their accumulation in K562 cells. Furthermore, EFL1 sensitized the apoptosis triggered by vincristine in K562/ADR cells via mitochondrial pathway, as confirmed by Annexin V-FITC/PI detection and western blot. At the same time, EFL1 did not influence the apoptosis induced by vincristine in K562 cells. Western blot results showed that EFL1 did not affect the phosphorylation level of AKT and ERK in K562 and K562/ADR cells. Finally, EFL1 did not down-regulate protein expression of ABCB1.

Original language	English
Pages (from-to)	12793-12808
Number of pages	16
Journal	Molecules
Volume	18
Issue number	10
DOIs	https://doi.org/10.3390/molecules181012793
Publication status	Published - Oct 2013

Scopus Subject Areas

Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry

User-Defined Keywords

ABCB1
Apoptosis
Euphorbia factor L1
Multidrug resistance

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/molecules181012793

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title = "Apoptosis sensitization by euphorbia factor L1 in ABCB1-mediated multidrug resistant K562/ADR cells",

abstract = "In this article, reversal activities of Euphorbia factor L1 (EFL1) against ABCB1-mediated multidrug resistance (MDR) and apoptosis sensitization in K562/ADR cells are reported. EFL1 decreased the IC50 values of anticancer agents in K562/ADR cells over-expressing ABCB1. However, EFL1 did not affect the IC50 values of anticancer agents in sensitive K562 cells. Additionally, EFL1 increased the intracellular accumulation of rhodamine 123 and doxorubicin in K562/ADR cells without affecting their accumulation in K562 cells. Furthermore, EFL1 sensitized the apoptosis triggered by vincristine in K562/ADR cells via mitochondrial pathway, as confirmed by Annexin V-FITC/PI detection and western blot. At the same time, EFL1 did not influence the apoptosis induced by vincristine in K562 cells. Western blot results showed that EFL1 did not affect the phosphorylation level of AKT and ERK in K562 and K562/ADR cells. Finally, EFL1 did not down-regulate protein expression of ABCB1.",

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author = "Zhang, {Jian Ye} and Lin, {Min Ting} and Tao YI and Tang, {Yi Na} and Fan, {Lan Lan} and He, {Xi Cheng} and Zhongzhen ZHAO and Hubiao CHEN",

note = "This work was supported by the grants from Guangdong Science and Technology Department & Guangdong Academy of Traditional Chinese Medicine (2012A032500021), research Project of Guangzhou Education Bureau (10A167) and funding from Guangzhou Science and Technology Program (2013J4100033). ",

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T1 - Apoptosis sensitization by euphorbia factor L1 in ABCB1-mediated multidrug resistant K562/ADR cells

AU - Zhang, Jian Ye

AU - Lin, Min Ting

AU - YI, Tao

AU - Tang, Yi Na

AU - Fan, Lan Lan

AU - He, Xi Cheng

AU - ZHAO, Zhongzhen

AU - CHEN, Hubiao

N1 - This work was supported by the grants from Guangdong Science and Technology Department & Guangdong Academy of Traditional Chinese Medicine (2012A032500021), research Project of Guangzhou Education Bureau (10A167) and funding from Guangzhou Science and Technology Program (2013J4100033).

PY - 2013/10

Y1 - 2013/10

N2 - In this article, reversal activities of Euphorbia factor L1 (EFL1) against ABCB1-mediated multidrug resistance (MDR) and apoptosis sensitization in K562/ADR cells are reported. EFL1 decreased the IC50 values of anticancer agents in K562/ADR cells over-expressing ABCB1. However, EFL1 did not affect the IC50 values of anticancer agents in sensitive K562 cells. Additionally, EFL1 increased the intracellular accumulation of rhodamine 123 and doxorubicin in K562/ADR cells without affecting their accumulation in K562 cells. Furthermore, EFL1 sensitized the apoptosis triggered by vincristine in K562/ADR cells via mitochondrial pathway, as confirmed by Annexin V-FITC/PI detection and western blot. At the same time, EFL1 did not influence the apoptosis induced by vincristine in K562 cells. Western blot results showed that EFL1 did not affect the phosphorylation level of AKT and ERK in K562 and K562/ADR cells. Finally, EFL1 did not down-regulate protein expression of ABCB1.

AB - In this article, reversal activities of Euphorbia factor L1 (EFL1) against ABCB1-mediated multidrug resistance (MDR) and apoptosis sensitization in K562/ADR cells are reported. EFL1 decreased the IC50 values of anticancer agents in K562/ADR cells over-expressing ABCB1. However, EFL1 did not affect the IC50 values of anticancer agents in sensitive K562 cells. Additionally, EFL1 increased the intracellular accumulation of rhodamine 123 and doxorubicin in K562/ADR cells without affecting their accumulation in K562 cells. Furthermore, EFL1 sensitized the apoptosis triggered by vincristine in K562/ADR cells via mitochondrial pathway, as confirmed by Annexin V-FITC/PI detection and western blot. At the same time, EFL1 did not influence the apoptosis induced by vincristine in K562 cells. Western blot results showed that EFL1 did not affect the phosphorylation level of AKT and ERK in K562 and K562/ADR cells. Finally, EFL1 did not down-regulate protein expression of ABCB1.

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Apoptosis sensitization by euphorbia factor L1 in ABCB1-mediated multidrug resistant K562/ADR cells

Abstract

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