TY - JOUR
T1 - Antioxidant responses and lipid peroxidation in gills and hepatopancreas of the mussel Perna viridis upon exposure to the red-tide organism Chattonella marina and hydrogen peroxide
AU - Kwok, Chung Ting
AU - van de Merwe, Jason P.
AU - Chiu, Jill M.Y.
AU - Wu, Rudolf S.S.
N1 - Funding Information:
The manuscript benefited greatly from the comments and suggestions of the anonymous reviewers. The work described in this paper was supported by a CERG grant and an AoE grant from the University Grants Committee of the Hong Kong Special Administrative Region, China to R.S.S. Wu (Project Nos. 9040864 and AoE/P-04/2004).[SS]
Publisher copyright:
Copyright © 2011 Elsevier B.V. All rights reserved.
PY - 2012/1
Y1 - 2012/1
N2 - In this study, we investigated the production of hydrogen
peroxide (H2O2) by the red-tide organism Chattonella
marina. Subsequently, we examined the antioxidant responses as well as
lipid peroxidation in gills and hepatopancreas of the mussel Perna
viridis upon exposure to C. marina at environmentally
realistic concentrations (103 and 104 cells ml−1).
Despite the extracellular levels of H2O2 generated
were up to ∼0.5 (at 103 cells ml−1)
and 20 μM (at 104 cells ml−1), no
significant differences could be observed in any of the examined biochemical
parameters (i.e. catalase (CAT), glutathione (GSH), superoxide dismutase (SOD),
glutathione peroxidase (GPx), glutathione-S-transferase (GST), glutathione
reductase (GR) and lipid peroxidation (LPO)) in mussels between treatment and
control groups. In another experiment, where mussels were exposed to H2O2,
no responses were induced at the concentration up to 0.5 mM. These data collectively
indicate that the production of H2O2 by C.
marina is not high enough to elicit antioxidant responses in mussels.
As such, reactive oxygen species (ROS) is unlikely to be an important
toxicological mechanism of C. marina.
AB - In this study, we investigated the production of hydrogen
peroxide (H2O2) by the red-tide organism Chattonella
marina. Subsequently, we examined the antioxidant responses as well as
lipid peroxidation in gills and hepatopancreas of the mussel Perna
viridis upon exposure to C. marina at environmentally
realistic concentrations (103 and 104 cells ml−1).
Despite the extracellular levels of H2O2 generated
were up to ∼0.5 (at 103 cells ml−1)
and 20 μM (at 104 cells ml−1), no
significant differences could be observed in any of the examined biochemical
parameters (i.e. catalase (CAT), glutathione (GSH), superoxide dismutase (SOD),
glutathione peroxidase (GPx), glutathione-S-transferase (GST), glutathione
reductase (GR) and lipid peroxidation (LPO)) in mussels between treatment and
control groups. In another experiment, where mussels were exposed to H2O2,
no responses were induced at the concentration up to 0.5 mM. These data collectively
indicate that the production of H2O2 by C.
marina is not high enough to elicit antioxidant responses in mussels.
As such, reactive oxygen species (ROS) is unlikely to be an important
toxicological mechanism of C. marina.
KW - Bivalves
KW - Harmful algal blooms
KW - Marine invertebrates
KW - Raphidophyceae
KW - Reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=84855236239&partnerID=8YFLogxK
U2 - 10.1016/j.hal.2011.10.001
DO - 10.1016/j.hal.2011.10.001
M3 - Journal article
AN - SCOPUS:84855236239
SN - 1568-9883
VL - 13
SP - 40
EP - 46
JO - Harmful Algae
JF - Harmful Algae
ER -