TY - JOUR
T1 - Antidiabetic activity and potential mechanism of amentoflavone in diabetic mice
AU - Su, Chengfu
AU - Yang, Chuanbin
AU - Gong, Man
AU - Ke, Yingying
AU - Yuan, Peipei
AU - Wang, Xiaolan
AU - LI, Min
AU - Zheng, Xiaoke
AU - Feng, Weisheng
N1 - Funding Information:
This research was funded by the NSFC (National Natural Science Foundation of China), grant number 81073034.
Funding Information:
Funding: This research was funded by the NSFC (National Natural Science Foundation of China), grant number 81073034.
PY - 2019/6/11
Y1 - 2019/6/11
N2 - Aim: To investigate the anti-diabetic activity of a mentoflavone (AME) indiabetic mice, and to explore the potential mechanisms. Methods: Diabetic mice induced by high fat diet and streptozotocin were administered with amentoflavone for 8 weeks. Biochemical indexes were tested to evaluate its anti-diabetic effect. Hepatic steatosis, the histopathology change of the pancreas was evaluated. The activity of glucose metabolic enzymes, the expression of Akt and pAkt, and the glucose transporter type 4 (GLUT4) immunoreactivity were detected. Results: AME decreased the level of glucose, total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and glucagon, and increased the levels of high density lipoprotein cholesterol (HDL-C) and insulin. Additionally, AME increased the activity of glucokinase (GCK), phosphofructokinase-1 (PFK-1), and pyruvate kinase (PK), and inhibited the activity of glycogen synthase kinase-3 (GSK-3), phosphoenolpyruvate carboxykinase (PEPCK), and glucose-6-phosphatase (G-6-Pase). Mechanistically, AME increased superoxide dismutase (SOD), decreased malondialdehyde (MDA), activation of several key signaling molecules including pAkt (Ser473), and increased the translocation to the sedimenting membranes of GLUT4 in skeletal muscle tissue. Conclusions: AME exerted anti-diabetic effects by regulating glucose and lipid metabolism, perhaps via anti-oxidant effects and activating the PI3K/Akt pathway. Our study provided novel insight into the role and underlying mechanisms of AME in diabetes.
AB - Aim: To investigate the anti-diabetic activity of a mentoflavone (AME) indiabetic mice, and to explore the potential mechanisms. Methods: Diabetic mice induced by high fat diet and streptozotocin were administered with amentoflavone for 8 weeks. Biochemical indexes were tested to evaluate its anti-diabetic effect. Hepatic steatosis, the histopathology change of the pancreas was evaluated. The activity of glucose metabolic enzymes, the expression of Akt and pAkt, and the glucose transporter type 4 (GLUT4) immunoreactivity were detected. Results: AME decreased the level of glucose, total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and glucagon, and increased the levels of high density lipoprotein cholesterol (HDL-C) and insulin. Additionally, AME increased the activity of glucokinase (GCK), phosphofructokinase-1 (PFK-1), and pyruvate kinase (PK), and inhibited the activity of glycogen synthase kinase-3 (GSK-3), phosphoenolpyruvate carboxykinase (PEPCK), and glucose-6-phosphatase (G-6-Pase). Mechanistically, AME increased superoxide dismutase (SOD), decreased malondialdehyde (MDA), activation of several key signaling molecules including pAkt (Ser473), and increased the translocation to the sedimenting membranes of GLUT4 in skeletal muscle tissue. Conclusions: AME exerted anti-diabetic effects by regulating glucose and lipid metabolism, perhaps via anti-oxidant effects and activating the PI3K/Akt pathway. Our study provided novel insight into the role and underlying mechanisms of AME in diabetes.
KW - Biochemical indexes
KW - Glucose metabolic enzyme
KW - GLUT4 translocation
KW - PI3K/Akt
UR - http://www.scopus.com/inward/record.url?scp=85067353943&partnerID=8YFLogxK
U2 - 10.3390/molecules24112184
DO - 10.3390/molecules24112184
M3 - Article
C2 - 31212585
AN - SCOPUS:85067353943
SN - 1420-3049
VL - 24
JO - Molecules
JF - Molecules
IS - 11
M1 - 2184
ER -
