Antiangiogenic activity of a concentrated effective microorganism fermentation extract

Chung Hin CHUI, Roberto Gambari, Fung Y.I. Lau, Desmond Kwok Po Hau, Raymond Siu Ming Wong, Gregory Yin Ming Cheng, Stanton Hon Lung Kok, Teruo Higa, Bin Ke, Albert Sun Chi Chan, David W F FONG, Johnny Cheuk On Tang

Research output: Contribution to journalJournal articlepeer-review

8 Citations (Scopus)


We have previously demonstrated the possible growth inhibitory activity of both first generation of the effective microorganism fermentation extract (EM-X) as well as the second generation (EM-X2) on cancer cell lines in vitro. The possible anti-angiogenic potential of EM-X has not been reported. Herein we show that using the concentrated EM-X, the growth of human umbilical cord endothelial cells (HUCE) was significantly inhibited in vitro. Enzyme linked immunosorbent assay suggested that the concentrated EM-X is able to reduce the level of vascular endothelial growth factor (VEGF) from Hep3B hepatocellular carcinoma (HCC) cells. The conditioned culture medium obtained from the concentrated EM-X incubated Hep3B HCC cells possessed significant antiproliferative effect on the HUCE cells. Moreover, in vivo chick chorioallantoic membrane assay further demon-strated that the concentrated EM-X is able to greatly inhibit the basic fibroblast growth factor induced angiogenesis from chick embryo experiment. We speculate that the anti-cancer potential of this concentrated EM-X involved growth inhibition on cancer cell and antiangiogenic effect on HUCE cells.

Original languageEnglish
Pages (from-to)975-979
Number of pages5
JournalInternational Journal of Molecular Medicine
Issue number5
Publication statusPublished - Nov 2006

Scopus Subject Areas

  • Genetics

User-Defined Keywords

  • Chick chorioallantoic membrane
  • Effective microorganism fermentation extract
  • Human umbilical cord endothelial cell


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