TY - JOUR
T1 - Antiangiogenic activity of a concentrated effective microorganism fermentation extract
AU - CHUI, Chung Hin
AU - Gambari, Roberto
AU - Lau, Fung Y.I.
AU - Hau, Desmond Kwok Po
AU - Wong, Raymond Siu Ming
AU - Cheng, Gregory Yin Ming
AU - Kok, Stanton Hon Lung
AU - Higa, Teruo
AU - Ke, Bin
AU - Chan, Albert Sun Chi
AU - FONG, David W F
AU - Tang, Johnny Cheuk On
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2006/11
Y1 - 2006/11
N2 - We have previously demonstrated the possible growth inhibitory activity of both first generation of the effective microorganism fermentation extract (EM-X) as well as the second generation (EM-X2) on cancer cell lines in vitro. The possible anti-angiogenic potential of EM-X has not been reported. Herein we show that using the concentrated EM-X, the growth of human umbilical cord endothelial cells (HUCE) was significantly inhibited in vitro. Enzyme linked immunosorbent assay suggested that the concentrated EM-X is able to reduce the level of vascular endothelial growth factor (VEGF) from Hep3B hepatocellular carcinoma (HCC) cells. The conditioned culture medium obtained from the concentrated EM-X incubated Hep3B HCC cells possessed significant antiproliferative effect on the HUCE cells. Moreover, in vivo chick chorioallantoic membrane assay further demon-strated that the concentrated EM-X is able to greatly inhibit the basic fibroblast growth factor induced angiogenesis from chick embryo experiment. We speculate that the anti-cancer potential of this concentrated EM-X involved growth inhibition on cancer cell and antiangiogenic effect on HUCE cells.
AB - We have previously demonstrated the possible growth inhibitory activity of both first generation of the effective microorganism fermentation extract (EM-X) as well as the second generation (EM-X2) on cancer cell lines in vitro. The possible anti-angiogenic potential of EM-X has not been reported. Herein we show that using the concentrated EM-X, the growth of human umbilical cord endothelial cells (HUCE) was significantly inhibited in vitro. Enzyme linked immunosorbent assay suggested that the concentrated EM-X is able to reduce the level of vascular endothelial growth factor (VEGF) from Hep3B hepatocellular carcinoma (HCC) cells. The conditioned culture medium obtained from the concentrated EM-X incubated Hep3B HCC cells possessed significant antiproliferative effect on the HUCE cells. Moreover, in vivo chick chorioallantoic membrane assay further demon-strated that the concentrated EM-X is able to greatly inhibit the basic fibroblast growth factor induced angiogenesis from chick embryo experiment. We speculate that the anti-cancer potential of this concentrated EM-X involved growth inhibition on cancer cell and antiangiogenic effect on HUCE cells.
KW - Chick chorioallantoic membrane
KW - Effective microorganism fermentation extract
KW - Human umbilical cord endothelial cell
UR - http://www.scopus.com/inward/record.url?scp=33847414563&partnerID=8YFLogxK
U2 - 10.3892/ijmm.18.5.975
DO - 10.3892/ijmm.18.5.975
M3 - Journal article
C2 - 17016630
AN - SCOPUS:33847414563
SN - 1107-3756
VL - 18
SP - 975
EP - 979
JO - International Journal of Molecular Medicine
JF - International Journal of Molecular Medicine
IS - 5
ER -