Anti-inflammatory activity of the extracts and fractions from Erigeron multiradiatus through bioassay-guided procedures

Pei Luo, Zhifeng Zhang, Tao YI, Hao Zhang, Xinfu Liu, Zhengji Mo*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

Ethnopharmacological relevance: Erigeron multiradiatus (Lindl.) Benth., an herb that grows in the alpine and subalpine meadow of Qinghai-Tibet plateau, has been widely used as a folk remedy by the native people for treatment of various inflammatory ailments. Aim of the study: In order to isolate and identify the active components of Erigeron multiradiatus for anti-inflammatory activity, a preliminary phytochemical study and a bioassay-guided fractionation and purification process was performed. Material and Methods: The dry whole plant Erigeron multiradiatus was extracted with 50% ethanol and then separated into CHCl3, n-BuOH, and aqueous fractions. The anti-inflammatory activities of each fraction were investigated using two in vivo inflammation models. Results: These results exhibited varying degrees of anti-inflammatory activities and the n-BuOH fraction showed the strongest anti-inflammatory activities. The n-BuOH fraction was then subjected to separation and purification using macroporous resins column chromatography and Sephadex LH-20 leading to two flavonoids glucuronides identified as scutellarein-7-O-β-glucuronide and apigenin-7-O-β-glucuronide. Furthermore, LC-MS/MS identification and quantification of isolated compounds were also performed. Conclusion: Scutellarein-7-O-β-glucuronide and apigenin-7-O-β-glucuronide were considered as major components and principally responsible for the anti-inflammatory activity of Erigeron multiradiatus. Thus the results of our study provide a scientific basis for the utilization of Erigeron multiradiatus in traditional Tibetan medicine. Crown

Original languageEnglish
Pages (from-to)232-237
Number of pages6
JournalJournal of Ethnopharmacology
Volume119
Issue number2
DOIs
Publication statusPublished - 26 Sep 2008

Scopus Subject Areas

  • Pharmacology
  • Drug Discovery

User-Defined Keywords

  • Anti-inflammatory activity
  • Erigeron multiradiatus
  • Flavonoids
  • LC-MS/MS

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