TY - JOUR
T1 - Anti-inflammatory activity of the extracts and fractions from Erigeron multiradiatus through bioassay-guided procedures
AU - Luo, Pei
AU - Zhang, Zhifeng
AU - YI, Tao
AU - Zhang, Hao
AU - Liu, Xinfu
AU - Mo, Zhengji
N1 - Funding Information:
The authors thank the Bureau of Science and Technology of Sichuan Province for the support of the applied fundamental study (No. 2006Z08—081). Thanks are also due to The Ministry of National Education Doctoral Fund (No. 20020610089) for providing financial support.
PY - 2008/9/26
Y1 - 2008/9/26
N2 - Ethnopharmacological relevance: Erigeron multiradiatus (Lindl.) Benth., an herb that grows in the alpine and subalpine meadow of Qinghai-Tibet plateau, has been widely used as a folk remedy by the native people for treatment of various inflammatory ailments. Aim of the study: In order to isolate and identify the active components of Erigeron multiradiatus for anti-inflammatory activity, a preliminary phytochemical study and a bioassay-guided fractionation and purification process was performed. Material and Methods: The dry whole plant Erigeron multiradiatus was extracted with 50% ethanol and then separated into CHCl3, n-BuOH, and aqueous fractions. The anti-inflammatory activities of each fraction were investigated using two in vivo inflammation models. Results: These results exhibited varying degrees of anti-inflammatory activities and the n-BuOH fraction showed the strongest anti-inflammatory activities. The n-BuOH fraction was then subjected to separation and purification using macroporous resins column chromatography and Sephadex LH-20 leading to two flavonoids glucuronides identified as scutellarein-7-O-β-glucuronide and apigenin-7-O-β-glucuronide. Furthermore, LC-MS/MS identification and quantification of isolated compounds were also performed. Conclusion: Scutellarein-7-O-β-glucuronide and apigenin-7-O-β-glucuronide were considered as major components and principally responsible for the anti-inflammatory activity of Erigeron multiradiatus. Thus the results of our study provide a scientific basis for the utilization of Erigeron multiradiatus in traditional Tibetan medicine. Crown
AB - Ethnopharmacological relevance: Erigeron multiradiatus (Lindl.) Benth., an herb that grows in the alpine and subalpine meadow of Qinghai-Tibet plateau, has been widely used as a folk remedy by the native people for treatment of various inflammatory ailments. Aim of the study: In order to isolate and identify the active components of Erigeron multiradiatus for anti-inflammatory activity, a preliminary phytochemical study and a bioassay-guided fractionation and purification process was performed. Material and Methods: The dry whole plant Erigeron multiradiatus was extracted with 50% ethanol and then separated into CHCl3, n-BuOH, and aqueous fractions. The anti-inflammatory activities of each fraction were investigated using two in vivo inflammation models. Results: These results exhibited varying degrees of anti-inflammatory activities and the n-BuOH fraction showed the strongest anti-inflammatory activities. The n-BuOH fraction was then subjected to separation and purification using macroporous resins column chromatography and Sephadex LH-20 leading to two flavonoids glucuronides identified as scutellarein-7-O-β-glucuronide and apigenin-7-O-β-glucuronide. Furthermore, LC-MS/MS identification and quantification of isolated compounds were also performed. Conclusion: Scutellarein-7-O-β-glucuronide and apigenin-7-O-β-glucuronide were considered as major components and principally responsible for the anti-inflammatory activity of Erigeron multiradiatus. Thus the results of our study provide a scientific basis for the utilization of Erigeron multiradiatus in traditional Tibetan medicine. Crown
KW - Anti-inflammatory activity
KW - Erigeron multiradiatus
KW - Flavonoids
KW - LC-MS/MS
UR - http://www.scopus.com/inward/record.url?scp=51249087928&partnerID=8YFLogxK
U2 - 10.1016/j.jep.2008.06.035
DO - 10.1016/j.jep.2008.06.035
M3 - Journal article
C2 - 18678238
AN - SCOPUS:51249087928
SN - 0378-8741
VL - 119
SP - 232
EP - 237
JO - Journal of Ethnopharmacology
JF - Journal of Ethnopharmacology
IS - 2
ER -