TY - JOUR
T1 - Anti-inflammatory activity of polysaccharide from Schizophyllum commune as affected by ultrasonication
AU - Du, Bin
AU - Zeng, Huansong
AU - Yang, Yuedong
AU - BIAN, Zhaoxiang
AU - Xu, Baojun
N1 - Funding Information:
This research was jointly supported by two research grants ( R201327 and R201402 ) from Beijing Normal University-Hong Kong Baptist University United International College, China .
PY - 2016/10/1
Y1 - 2016/10/1
N2 - Ultrasound treatment was applied to modify the physicochemical properties of an exopolysaccharide from mycelial culture of Schizophyllum commune. Molecular weight (MW) degradation, viscosity and anti-inflammatory property of ultrasonic treated polysaccharide were optimized with response surface methodology. The best ultrasonic parameters were obtained with a three-variable-three-level Box-Behnken design. The optimized conditions for efficient anti-inflammatory activity are initial concentration at 0.4%, ultrasonic power at 600 W, and duration of ultrasonic irradiation for 9 min. Under these conditions, the nitric oxide inhibition rate was 95 ± 0.03% which agreed closely with the predicted value (96%). Average MW of polysaccharide decreased after ultrasonic treatments. The viscosity of degraded polysaccharide dropped compared with native polysaccharide. The anti-inflammatory activity was improved by ultrasound treatment. The results suggested that ultrasound treatment is an effective approach to decrease the MW of polysaccharide with high anti-inflammatory activity. Ultrasonic treatment is a viable modification technology for high MW polymer materials.
AB - Ultrasound treatment was applied to modify the physicochemical properties of an exopolysaccharide from mycelial culture of Schizophyllum commune. Molecular weight (MW) degradation, viscosity and anti-inflammatory property of ultrasonic treated polysaccharide were optimized with response surface methodology. The best ultrasonic parameters were obtained with a three-variable-three-level Box-Behnken design. The optimized conditions for efficient anti-inflammatory activity are initial concentration at 0.4%, ultrasonic power at 600 W, and duration of ultrasonic irradiation for 9 min. Under these conditions, the nitric oxide inhibition rate was 95 ± 0.03% which agreed closely with the predicted value (96%). Average MW of polysaccharide decreased after ultrasonic treatments. The viscosity of degraded polysaccharide dropped compared with native polysaccharide. The anti-inflammatory activity was improved by ultrasound treatment. The results suggested that ultrasound treatment is an effective approach to decrease the MW of polysaccharide with high anti-inflammatory activity. Ultrasonic treatment is a viable modification technology for high MW polymer materials.
KW - Anti-inflammatory
KW - Response surface methodology
KW - Ultrasonic treatment
UR - http://www.scopus.com/inward/record.url?scp=84973322295&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2016.05.052
DO - 10.1016/j.ijbiomac.2016.05.052
M3 - Journal article
C2 - 27189700
AN - SCOPUS:84973322295
SN - 0141-8130
VL - 91
SP - 100
EP - 105
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
ER -