TY - JOUR
T1 - Anti-fibrotic effect of trans-resveratrol on pancreatic stellate cells
AU - TSANG, Siu Wai
AU - ZHANG, Hongjie
AU - Lin, Zesi
AU - Mu, Huaixue
AU - BIAN, Zhaoxiang
N1 - Funding Information:
This work was supported by the Centre for Cancer and Inflammation Research, Hong Kong Baptist University, Hong Kong SAR, China and partially supported by the National Natural Science Foundation of China (young scientist project number 81400666). The authors gratefully acknowledge Prof. Robert Jaster for providing the LTC-14 cell line used in our in vitro experiments.
PY - 2015/4/1
Y1 - 2015/4/1
N2 - Trans-resveratrol, also known as 3,5,4'-trihydroxy-. trans-stilbene, is a natural stilbenoid found at high concentration in skins of red grapes and berries. Over the recent years, it has been reported with a variety of beneficial effects such as antioxidant, anti-aging and anti-inflammatory bioactivities; thus often utilized as an active substance in human and veterinary therapeutics. In the current study, we aimed to delineate the mechanism of its anti-fibrotic action by means of various biochemical assays, such as immunofluorescent staining, real-time polymerase chain reaction and Western blotting analyses in a cellular model, the LTC-14 cells, which retain essential characteristics and morphological features of primary pancreatic stellate cells (PSCs). Our results demonstrated that the application of trans-resveratrol as low as 10. μM notably suppressed the mRNA and protein levels of different fibrotic mediators namely alpha-smooth muscle actin, type I collagen and fibronectin in the LTC-14 cells stimulated with transforming growth factor-beta, a well recognized pro-fibrotic inducer. Importantly, the mechanism of the anti-fibrotic action of trans-resveratrol was associated with a decrease in nuclear factor-kappaB activation and protein kinase B phosphorylation. In conclusion, our finding suggests that trans-resveratrol may serve as a therapeutic or an adjuvant agent in anti-fibrotic approaches and/or PSC-relating pathologies.
AB - Trans-resveratrol, also known as 3,5,4'-trihydroxy-. trans-stilbene, is a natural stilbenoid found at high concentration in skins of red grapes and berries. Over the recent years, it has been reported with a variety of beneficial effects such as antioxidant, anti-aging and anti-inflammatory bioactivities; thus often utilized as an active substance in human and veterinary therapeutics. In the current study, we aimed to delineate the mechanism of its anti-fibrotic action by means of various biochemical assays, such as immunofluorescent staining, real-time polymerase chain reaction and Western blotting analyses in a cellular model, the LTC-14 cells, which retain essential characteristics and morphological features of primary pancreatic stellate cells (PSCs). Our results demonstrated that the application of trans-resveratrol as low as 10. μM notably suppressed the mRNA and protein levels of different fibrotic mediators namely alpha-smooth muscle actin, type I collagen and fibronectin in the LTC-14 cells stimulated with transforming growth factor-beta, a well recognized pro-fibrotic inducer. Importantly, the mechanism of the anti-fibrotic action of trans-resveratrol was associated with a decrease in nuclear factor-kappaB activation and protein kinase B phosphorylation. In conclusion, our finding suggests that trans-resveratrol may serve as a therapeutic or an adjuvant agent in anti-fibrotic approaches and/or PSC-relating pathologies.
KW - Anti-fibrotic agent
KW - Pancreatic fibrosis
KW - Pancreatic stellate cells
KW - Resveratrol
KW - Trans-resveratrol
UR - http://www.scopus.com/inward/record.url?scp=84928966722&partnerID=8YFLogxK
U2 - 10.1016/j.biopha.2015.02.013
DO - 10.1016/j.biopha.2015.02.013
M3 - Journal article
C2 - 25960221
AN - SCOPUS:84928966722
SN - 0753-3322
VL - 71
SP - 91
EP - 97
JO - Biomedicine and Pharmacotherapy
JF - Biomedicine and Pharmacotherapy
ER -