TY - JOUR
T1 - Antcamphorols A-K, Cytotoxic and ROS Scavenging Triterpenoids from Antrodia camphorata
AU - Li, Bin
AU - Kuang, Yi
AU - He, Jun Bin
AU - Tang, Rui
AU - Xu, Lu Lu
AU - Leung, Chung Hang
AU - MA, Edmond Dik Lung
AU - Qiao, Xue
AU - Ye, Min
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (81725023, 81891010/81891011, and 81921001), the Beijing Natural Science Foundation (JQ18027), and the National Mega-project for Innovative Drugs (2018ZX09711001-008-003). We wish to thank Mr. Jen-Yu Lo at Honest & Humble Biotechnology Co., Ltd. (New Taipei City, Taiwan) for kindly providing the dish-cultured samples.
PY - 2020/1/24
Y1 - 2020/1/24
N2 - Antrodia camphorata is a rare and valuable medicinal mushroom. In this work, 11 new triterpenoids, namely, antcamphorols A-K (1-11), together with 10 known triterpenoids, 12-21, were isolated from dish-cultured A. camphorata. Compound 1 is an unprecedented C31 lanostane-type triterpenoid featuring a methyl group at C-15 and a C-21-O-C-24 tetrahydropyran ring at C-17. Compounds 2-11 are ergostane-type triterpenoids, and they include two pairs of norergostanes 2-5. The structures of the new compounds were identified by NMR, 2D NMR, and HRESIMS data analyses. The absolute configurations of 1 and 6 were defined by X-ray diffraction data, and the absolute configuration at C-25 of 4 was determined by the modified Mosher's method. Compounds 7, 9, 10, 16, and 19 showed significant ROS scavenging activities (63.9-70.5% at 20 μM) in high-glucose-induced HUVECs. Compounds 3 and 8 exhibited moderate cytotoxic activities against U251 (IC50, 9.2 μM) and MCF-7 (IC50, 8.1 μM) human cancer cell lines, respectively.
AB - Antrodia camphorata is a rare and valuable medicinal mushroom. In this work, 11 new triterpenoids, namely, antcamphorols A-K (1-11), together with 10 known triterpenoids, 12-21, were isolated from dish-cultured A. camphorata. Compound 1 is an unprecedented C31 lanostane-type triterpenoid featuring a methyl group at C-15 and a C-21-O-C-24 tetrahydropyran ring at C-17. Compounds 2-11 are ergostane-type triterpenoids, and they include two pairs of norergostanes 2-5. The structures of the new compounds were identified by NMR, 2D NMR, and HRESIMS data analyses. The absolute configurations of 1 and 6 were defined by X-ray diffraction data, and the absolute configuration at C-25 of 4 was determined by the modified Mosher's method. Compounds 7, 9, 10, 16, and 19 showed significant ROS scavenging activities (63.9-70.5% at 20 μM) in high-glucose-induced HUVECs. Compounds 3 and 8 exhibited moderate cytotoxic activities against U251 (IC50, 9.2 μM) and MCF-7 (IC50, 8.1 μM) human cancer cell lines, respectively.
UR - http://www.scopus.com/inward/record.url?scp=85078384749&partnerID=8YFLogxK
U2 - 10.1021/acs.jnatprod.9b00580
DO - 10.1021/acs.jnatprod.9b00580
M3 - Journal article
C2 - 31891260
AN - SCOPUS:85078384749
SN - 0163-3864
VL - 83
SP - 45
EP - 54
JO - Journal of Natural Products
JF - Journal of Natural Products
IS - 1
ER -
