TY - JOUR
T1 - Analysis of urinary porphyrins by high performance liquid chromatography-electrospray ionization mass spectrometry
AU - Li, Jinhua
AU - CAI, Zongwei
AU - Xu, Shoufang
AU - Liao, Chunyang
AU - Song, Xingliang
AU - Chen, Lingxin
N1 - Funding Information:
Financial support from the National Natural Science Foundation of China (20975089, 20907039), Department of Science and Technology of Shandong Province of China (2008GG20005005, 2010GSF10222), the Natural Science Foundation of Shandong Province of China (Y2007B38), Yantai Research and Development Program (2007323), and the 100 Talents Program of the Chinese Academy of Sciences is gratefully acknowledged.
PY - 2011/9
Y1 - 2011/9
N2 - A high-performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC-ESI-MS) method was developed for the simultaneous separation and detection of six porphyrins, namely uroporphyrin (UP), heptacarboxylic acid porphyrin (HEPTAP), hexacarboxylic acid porphyrin (HEXAP), pentacarboxylic acid porphyrin (PENTAP), coproporphyrin (CP), and mesoporphyrin (MP), in human urine samples. The recoveries of the porphyrins in urine ranged from 84 to 108%. The intra- and inter-day precisions at spiked porphyrin concentrations were 4.0-9.7% and 5.5-15%, respectively. The limits of detection (LOD) ranged from 0.2 to 3nM. The developed method was successfully applied to the determination of endogenous porphyrins in human urine. UP, HEPTAP, and PENTAP were detected at levels of 42.4, 21.3, and 18.0nM, respectively. CP was detected as the predominant species at 135.6nM, wheres MP and HEXAP were not detected in the urine samples from healthy females. Significant increases of CP excretion in urine from liver disease patients were observed. This method proved potentially applicable for the routine diagnosis and treatment of porphyrin-related diseases.
AB - A high-performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC-ESI-MS) method was developed for the simultaneous separation and detection of six porphyrins, namely uroporphyrin (UP), heptacarboxylic acid porphyrin (HEPTAP), hexacarboxylic acid porphyrin (HEXAP), pentacarboxylic acid porphyrin (PENTAP), coproporphyrin (CP), and mesoporphyrin (MP), in human urine samples. The recoveries of the porphyrins in urine ranged from 84 to 108%. The intra- and inter-day precisions at spiked porphyrin concentrations were 4.0-9.7% and 5.5-15%, respectively. The limits of detection (LOD) ranged from 0.2 to 3nM. The developed method was successfully applied to the determination of endogenous porphyrins in human urine. UP, HEPTAP, and PENTAP were detected at levels of 42.4, 21.3, and 18.0nM, respectively. CP was detected as the predominant species at 135.6nM, wheres MP and HEXAP were not detected in the urine samples from healthy females. Significant increases of CP excretion in urine from liver disease patients were observed. This method proved potentially applicable for the routine diagnosis and treatment of porphyrin-related diseases.
KW - electrospray ionization
KW - high performance liquid chromatography
KW - human urine
KW - mass spectrometry
KW - porphyrins
KW - solid-phase extraction
UR - http://www.scopus.com/inward/record.url?scp=80052721346&partnerID=8YFLogxK
U2 - 10.1080/10826076.2011.575981
DO - 10.1080/10826076.2011.575981
M3 - Journal article
AN - SCOPUS:80052721346
SN - 1082-6076
VL - 34
SP - 1578
EP - 1593
JO - Journal of Liquid Chromatography and Related Technologies
JF - Journal of Liquid Chromatography and Related Technologies
IS - 15
ER -
