TY - JOUR
T1 - Analysis of different strategies adapted by two cassava cultivars in response to drought stress
T2 - Ensuring survival or continuing growth
AU - Zhao, Pingjuan
AU - Liu, Pei
AU - Shao, Jiaofang
AU - Li, Chunqiang
AU - Wang, Bin
AU - Guo, Xin
AU - YAN, Bin
AU - XIA, Yiji
AU - Peng, Ming
N1 - Funding Information:
This work was supported by the ‘973’ program of the Ministry of Science and Technology of China (grant no. 2010CB126600 to MP), the ‘863’ program of the Ministry of Science and Technology of China (grant no. 2012AA101204-2 to MP), the Research Grants Council of Hong Kong (grant no. HKBU1/CRF/10 to YX), the HKBU Strategic Development Fund (to YX), the Major Technology Project of Hainan Province (grant no. ZDZX2013023-1 to MP), and the International Science & Technology Cooperation Program of China (grant no. 2013DFA32020 to MP).
PY - 2015/3/1
Y1 - 2015/3/1
N2 - Cassava is one of the most drought-tolerant crops, however, the underlying mechanism for its ability to survive and produce under drought remains obscure. In this study, two cassava cultivars, SC124 and Arg7, were treated by gradually reducing the soil water content. Their responses to the drought stress were examined through their morphological and physiological traits and isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic analysis. SC124 plants adapted a 'survival' mode under mild drought stress as evidenced by early stomatal closure and a reduction in the levels of various photosynthetic proteins and photosynthetic capacity, resulting in early growth quiescence. In contrast, Arg7 plants underwent senescence of older leaves but continued to grow, although at a reduced rate, under mild drought. SC124 plants were more capable of surviving prolonged severe drought than Arg7. The iTRAQ analysis identified over 5000 cassava proteins. Among the drought-responsive proteins identified in the study were an aquaporin, myo-inositol 1-phosphate synthases, and a number of proteins involved in the antioxidant systems and secondary metabolism. Many proteins that might play a role in signalling or gene regulation were also identified as drought-responsive proteins, which included several protein kinases, two 14-3-3 proteins, several RNA-binding proteins and transcription factors, and two histone deacetylases. Our study also supports the notion that linamarin might play a role in nitrogen reallocation in cassava under drought.
AB - Cassava is one of the most drought-tolerant crops, however, the underlying mechanism for its ability to survive and produce under drought remains obscure. In this study, two cassava cultivars, SC124 and Arg7, were treated by gradually reducing the soil water content. Their responses to the drought stress were examined through their morphological and physiological traits and isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic analysis. SC124 plants adapted a 'survival' mode under mild drought stress as evidenced by early stomatal closure and a reduction in the levels of various photosynthetic proteins and photosynthetic capacity, resulting in early growth quiescence. In contrast, Arg7 plants underwent senescence of older leaves but continued to grow, although at a reduced rate, under mild drought. SC124 plants were more capable of surviving prolonged severe drought than Arg7. The iTRAQ analysis identified over 5000 cassava proteins. Among the drought-responsive proteins identified in the study were an aquaporin, myo-inositol 1-phosphate synthases, and a number of proteins involved in the antioxidant systems and secondary metabolism. Many proteins that might play a role in signalling or gene regulation were also identified as drought-responsive proteins, which included several protein kinases, two 14-3-3 proteins, several RNA-binding proteins and transcription factors, and two histone deacetylases. Our study also supports the notion that linamarin might play a role in nitrogen reallocation in cassava under drought.
KW - Cassava
KW - Cyanogenic glycosides
KW - Drought tolerance
KW - Drought-responsive protein
KW - Proteomics
KW - Stress response
UR - http://www.scopus.com/inward/record.url?scp=84924366086&partnerID=8YFLogxK
U2 - 10.1093/jxb/eru507
DO - 10.1093/jxb/eru507
M3 - Journal article
C2 - 25547914
AN - SCOPUS:84924366086
SN - 0022-0957
VL - 66
SP - 1477
EP - 1488
JO - Journal of Experimental Botany
JF - Journal of Experimental Botany
IS - 5
ER -