An ultrasensitive colorimeter assay strategy for p53 mutation assisted by nicking endonuclease signal amplification

Zhenyu Lin; Weiqiang Yang; Guiyun Zhang; Qida Liu; Bin Qiu; Zongwei CAI; Guonan Chen

doi:10.1039/c1cc13146j

An ultrasensitive colorimeter assay strategy for p53 mutation assisted by nicking endonuclease signal amplification

Zhenyu Lin^*, Weiqiang Yang, Guiyun Zhang, Qida Liu, Bin Qiu, Zongwei CAI, Guonan Chen

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

42 Citations (Scopus)

Abstract

A novel catalytic colorimetric assay assisted by nicking endonuclease signal amplification (NESA) was developed. With the signal amplification, the detection limit of the p53 target gene can be as low as 1 pM, which is nearly 5 orders of magnitude lower than that of other previously reported colorimetric DNA detection strategies based on catalytic DNAzyme.

Original language	English
Pages (from-to)	9069-9071
Number of pages	3
Journal	Chemical Communications
Volume	47
Issue number	32
DOIs	https://doi.org/10.1039/c1cc13146j
Publication status	Published - 28 Aug 2011

Scopus Subject Areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/c1cc13146j

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title = "An ultrasensitive colorimeter assay strategy for p53 mutation assisted by nicking endonuclease signal amplification",

abstract = "A novel catalytic colorimetric assay assisted by nicking endonuclease signal amplification (NESA) was developed. With the signal amplification, the detection limit of the p53 target gene can be as low as 1 pM, which is nearly 5 orders of magnitude lower than that of other previously reported colorimetric DNA detection strategies based on catalytic DNAzyme.",

author = "Zhenyu Lin and Weiqiang Yang and Guiyun Zhang and Qida Liu and Bin Qiu and Zongwei CAI and Guonan Chen",

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AU - Lin, Zhenyu

AU - Yang, Weiqiang

AU - Zhang, Guiyun

AU - Liu, Qida

AU - Qiu, Bin

AU - CAI, Zongwei

AU - Chen, Guonan

PY - 2011/8/28

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N2 - A novel catalytic colorimetric assay assisted by nicking endonuclease signal amplification (NESA) was developed. With the signal amplification, the detection limit of the p53 target gene can be as low as 1 pM, which is nearly 5 orders of magnitude lower than that of other previously reported colorimetric DNA detection strategies based on catalytic DNAzyme.

AB - A novel catalytic colorimetric assay assisted by nicking endonuclease signal amplification (NESA) was developed. With the signal amplification, the detection limit of the p53 target gene can be as low as 1 pM, which is nearly 5 orders of magnitude lower than that of other previously reported colorimetric DNA detection strategies based on catalytic DNAzyme.

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DO - 10.1039/c1cc13146j

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JO - Chemical Communications

JF - Chemical Communications

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An ultrasensitive colorimeter assay strategy for p53 mutation assisted by nicking endonuclease signal amplification

Abstract

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