An ultrasensitive colorimeter assay strategy for p53 mutation assisted by nicking endonuclease signal amplification

Zhenyu Lin; Weiqiang Yang; Guiyun Zhang; Qida Liu; Bin Qiu; Zongwei CAI; Guonan Chen

doi:10.1039/c1cc13146j

An ultrasensitive colorimeter assay strategy for p53 mutation assisted by nicking endonuclease signal amplification

Zhenyu Lin^*, Weiqiang Yang, Guiyun Zhang, Qida Liu, Bin Qiu, Zongwei CAI, Guonan Chen

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

47 Citations (Scopus)

Abstract

A novel catalytic colorimetric assay assisted by nicking endonuclease signal amplification (NESA) was developed. With the signal amplification, the detection limit of the p53 target gene can be as low as 1 pM, which is nearly 5 orders of magnitude lower than that of other previously reported colorimetric DNA detection strategies based on catalytic DNAzyme.

Original language	English
Pages (from-to)	9069-9071
Number of pages	3
Journal	Chemical Communications
Volume	47
Issue number	32
DOIs	https://doi.org/10.1039/c1cc13146j
Publication status	Published - 28 Aug 2011

Scopus Subject Areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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title = "An ultrasensitive colorimeter assay strategy for p53 mutation assisted by nicking endonuclease signal amplification",

abstract = "A novel catalytic colorimetric assay assisted by nicking endonuclease signal amplification (NESA) was developed. With the signal amplification, the detection limit of the p53 target gene can be as low as 1 pM, which is nearly 5 orders of magnitude lower than that of other previously reported colorimetric DNA detection strategies based on catalytic DNAzyme.",

author = "Zhenyu Lin and Weiqiang Yang and Guiyun Zhang and Qida Liu and Bin Qiu and Zongwei CAI and Guonan Chen",

note = "This project was financially supported by the National Basic Research Program of China (No.2010CB732403), National Nature Sciences Funding of China (20928005, 41076059, 20905013, 10802062) and by the Program for New Century Excellent Talents in Fujian Province University (JA10011).",

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doi = "10.1039/c1cc13146j",

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AU - Lin, Zhenyu

AU - Yang, Weiqiang

AU - Zhang, Guiyun

AU - Liu, Qida

AU - Qiu, Bin

AU - CAI, Zongwei

AU - Chen, Guonan

N1 - This project was financially supported by the National Basic Research Program of China (No.2010CB732403), National Nature Sciences Funding of China (20928005, 41076059, 20905013, 10802062) and by the Program for New Century Excellent Talents in Fujian Province University (JA10011).

PY - 2011/8/28

Y1 - 2011/8/28

N2 - A novel catalytic colorimetric assay assisted by nicking endonuclease signal amplification (NESA) was developed. With the signal amplification, the detection limit of the p53 target gene can be as low as 1 pM, which is nearly 5 orders of magnitude lower than that of other previously reported colorimetric DNA detection strategies based on catalytic DNAzyme.

AB - A novel catalytic colorimetric assay assisted by nicking endonuclease signal amplification (NESA) was developed. With the signal amplification, the detection limit of the p53 target gene can be as low as 1 pM, which is nearly 5 orders of magnitude lower than that of other previously reported colorimetric DNA detection strategies based on catalytic DNAzyme.

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U2 - 10.1039/c1cc13146j

DO - 10.1039/c1cc13146j

M3 - Journal article

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VL - 47

SP - 9069

EP - 9071

JO - Chemical Communications

JF - Chemical Communications

IS - 32

ER -

An ultrasensitive colorimeter assay strategy for p53 mutation assisted by nicking endonuclease signal amplification

Abstract

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