An optical glucose biosensor based on glucose oxidase immobilized on a swim bladder membrane

Zaide Zhou, Lina Qiao, Peng Zhang, Dan Xiao*, Martin M F CHOI

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

An optical glucose biosensor using a swim bladder membrane as an enzyme immobilization platform and an oxygen-sensitive membrane as an optical oxygen transducer has been developed. During the enzymatic reaction, glucose is oxidized by glucose oxidase with a concomitant consumption of dissolved oxygen resulting in an increase in the fluorescence intensity of the optical oxygen transducer. The fluorescence intensity is directly related to the glucose concentration. The effects of pH, temperature, buffer concentration, and selectivity have been studied in detail. The immobilized enzyme retained 80% of its initial activity after being kept for more than 10 months at 4°C. The glucose biosensor has been successfully applied to the determination of glucose content in human blood serum and urine samples.

Original languageEnglish
Pages (from-to)673-679
Number of pages7
JournalAnalytical and Bioanalytical Chemistry
Volume383
Issue number4
DOIs
Publication statusPublished - Oct 2005

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry

User-Defined Keywords

  • Enzyme immobilization
  • Glucose biosensor
  • Oxygen-sensitive membrane
  • Swim bladder membrane

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