An oligosaccharide marker for rapid authentication of edible bird's nest

Li Feng Li; Hui Yuan Cheng; Jun Lang; Tin Long Wong; Ji Xia Zhang; Wen Jie Wu; Quan Wei Zhang; Hau Yee Kong; Check Hei Lai; Hau Yee Fung; Man Liu; Wan Rong Bao; Chu Ying Huo; Hong Ming Zheng; Qiu Ke Hou; Su Ping Bai; Quan Bin Han

doi:10.1016/j.foodchem.2022.135334

An oligosaccharide marker for rapid authentication of edible bird's nest

Li Feng Li
, Hui Yuan Cheng
, Jun Lang
, Tin Long Wong
, Ji Xia Zhang
, Wen Jie Wu
, Quan Wei Zhang
, Hau Yee Kong
, Check Hei Lai
, Hau Yee Fung
, Man Liu
, Wan Rong Bao
, Chu Ying Huo
, Hong Ming Zheng
, Qiu Ke Hou
, Su Ping Bai
, Quan Bin Han^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

5 Citations (Scopus)

Abstract

Edible bird's nest (EBN) is a popular and expensive food material. The limited supply and great demand result in the use of adulterants. The authenticity concern is raised due to the lack of appropriate quality markers. Herein, this study aims to provide a specific oligosaccharide marker for rapid EBN authentication. Comparing the benzocaine (ABEE)-labeled saccharide profiles of multiple batches of EBN and adulterants indicates seven unique EBN oligosaccharides. The most abundant one, named BNM001, was selected as a marker and characterized to be Neu5Ac (2–3) Gal by MS and NMR spectra. This new oligosaccharide marker enables a rapid authentication of EBN within 10 min. ABEE labelling of this marker further upgraded the accuracy and sensitivity of the LC-qTOF-MS quantitative analysis. The relative marker content was associated with the quality of EBN products. These results suggest a specific and efficient quality marker for rapid authentication of EBN and related products.

Original language	English
Article number	135334
Number of pages	9
Journal	Food Chemistry
Volume	409
Early online date	30 Dec 2022
DOIs	https://doi.org/10.1016/j.foodchem.2022.135334
Publication status	Published - 30 May 2023

User-Defined Keywords

Authentication
Edible bird's nest
LC–MS
Oligosaccharide
Quality marker

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.foodchem.2022.135334

Full TextAccepted author manuscript, 1.07 MBLicence: CC BY-NC-ND

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Li, L. F., Cheng, H. Y., Lang, J., Wong, T. L., Zhang, J. X., Wu, W. J., Zhang, Q. W., Kong, H. Y., Lai, C. H., Fung, H. Y., Liu, M., Bao, W. R., Huo, C. Y., Zheng, H. M., Hou, Q. K., Bai, S. P., & Han, Q. B. (2023). An oligosaccharide marker for rapid authentication of edible bird's nest. Food Chemistry, 409, Article 135334. https://doi.org/10.1016/j.foodchem.2022.135334

@article{ce540c794a9d4611bdff408fc36cc47e,

title = "An oligosaccharide marker for rapid authentication of edible bird's nest",

abstract = "Edible bird's nest (EBN) is a popular and expensive food material. The limited supply and great demand result in the use of adulterants. The authenticity concern is raised due to the lack of appropriate quality markers. Herein, this study aims to provide a specific oligosaccharide marker for rapid EBN authentication. Comparing the benzocaine (ABEE)-labeled saccharide profiles of multiple batches of EBN and adulterants indicates seven unique EBN oligosaccharides. The most abundant one, named BNM001, was selected as a marker and characterized to be Neu5Ac (2–3) Gal by MS and NMR spectra. This new oligosaccharide marker enables a rapid authentication of EBN within 10 min. ABEE labelling of this marker further upgraded the accuracy and sensitivity of the LC-qTOF-MS quantitative analysis. The relative marker content was associated with the quality of EBN products. These results suggest a specific and efficient quality marker for rapid authentication of EBN and related products.",

keywords = "Authentication, Edible bird's nest, LC–MS, Oligosaccharide, Quality marker",

author = "Li, \{Li Feng\} and Cheng, \{Hui Yuan\} and Jun Lang and Wong, \{Tin Long\} and Zhang, \{Ji Xia\} and Wu, \{Wen Jie\} and Zhang, \{Quan Wei\} and Kong, \{Hau Yee\} and Lai, \{Check Hei\} and Fung, \{Hau Yee\} and Man Liu and Bao, \{Wan Rong\} and Huo, \{Chu Ying\} and Zheng, \{Hong Ming\} and Hou, \{Qiu Ke\} and Bai, \{Su Ping\} and Han, \{Quan Bin\}",

note = "Funding Information: This work was funded and supported by HKSAR Innovation and Technology Fund (ITF), Tier 3, ITS/311/09, General Research Fund (12100615, 22100014, 12100818), UGC Research Matching Grant Scheme 2019-1-10, 2019-1-14, 2019-2-06, Health Medical Research Fund (11122531, 14150521, 17182681), National Natural Sciences Foundation in China (81473341), the Science and Technology Project of Shenzhen (JCYJ20160531193812867), the Key-Area Research and Development Program of Guangdong Province (2020B1111110007), Hong Kong Baptist University (RC-Start-up Grants, MPCF-004-2021/2022, MPCF-001-2016/2017, RC-IRMS/14-15/06, FRG2/17-18/060 and FRG2/16-17/002), and Vincent \& Lily Woo Foundation. Publisher Copyright: {\textcopyright} 2022 Elsevier Ltd.",

year = "2023",

month = may,

day = "30",

doi = "10.1016/j.foodchem.2022.135334",

language = "English",

volume = "409",

journal = "Food Chemistry",

issn = "0308-8146",

publisher = "Elsevier Ltd.",

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TY - JOUR

T1 - An oligosaccharide marker for rapid authentication of edible bird's nest

AU - Li, Li Feng

AU - Cheng, Hui Yuan

AU - Lang, Jun

AU - Wong, Tin Long

AU - Zhang, Ji Xia

AU - Wu, Wen Jie

AU - Zhang, Quan Wei

AU - Kong, Hau Yee

AU - Lai, Check Hei

AU - Fung, Hau Yee

AU - Liu, Man

AU - Bao, Wan Rong

AU - Huo, Chu Ying

AU - Zheng, Hong Ming

AU - Hou, Qiu Ke

AU - Bai, Su Ping

AU - Han, Quan Bin

N1 - Funding Information: This work was funded and supported by HKSAR Innovation and Technology Fund (ITF), Tier 3, ITS/311/09, General Research Fund (12100615, 22100014, 12100818), UGC Research Matching Grant Scheme 2019-1-10, 2019-1-14, 2019-2-06, Health Medical Research Fund (11122531, 14150521, 17182681), National Natural Sciences Foundation in China (81473341), the Science and Technology Project of Shenzhen (JCYJ20160531193812867), the Key-Area Research and Development Program of Guangdong Province (2020B1111110007), Hong Kong Baptist University (RC-Start-up Grants, MPCF-004-2021/2022, MPCF-001-2016/2017, RC-IRMS/14-15/06, FRG2/17-18/060 and FRG2/16-17/002), and Vincent & Lily Woo Foundation. Publisher Copyright: © 2022 Elsevier Ltd.

PY - 2023/5/30

Y1 - 2023/5/30

N2 - Edible bird's nest (EBN) is a popular and expensive food material. The limited supply and great demand result in the use of adulterants. The authenticity concern is raised due to the lack of appropriate quality markers. Herein, this study aims to provide a specific oligosaccharide marker for rapid EBN authentication. Comparing the benzocaine (ABEE)-labeled saccharide profiles of multiple batches of EBN and adulterants indicates seven unique EBN oligosaccharides. The most abundant one, named BNM001, was selected as a marker and characterized to be Neu5Ac (2–3) Gal by MS and NMR spectra. This new oligosaccharide marker enables a rapid authentication of EBN within 10 min. ABEE labelling of this marker further upgraded the accuracy and sensitivity of the LC-qTOF-MS quantitative analysis. The relative marker content was associated with the quality of EBN products. These results suggest a specific and efficient quality marker for rapid authentication of EBN and related products.

AB - Edible bird's nest (EBN) is a popular and expensive food material. The limited supply and great demand result in the use of adulterants. The authenticity concern is raised due to the lack of appropriate quality markers. Herein, this study aims to provide a specific oligosaccharide marker for rapid EBN authentication. Comparing the benzocaine (ABEE)-labeled saccharide profiles of multiple batches of EBN and adulterants indicates seven unique EBN oligosaccharides. The most abundant one, named BNM001, was selected as a marker and characterized to be Neu5Ac (2–3) Gal by MS and NMR spectra. This new oligosaccharide marker enables a rapid authentication of EBN within 10 min. ABEE labelling of this marker further upgraded the accuracy and sensitivity of the LC-qTOF-MS quantitative analysis. The relative marker content was associated with the quality of EBN products. These results suggest a specific and efficient quality marker for rapid authentication of EBN and related products.

KW - Authentication

KW - Edible bird's nest

KW - LC–MS

KW - Oligosaccharide

KW - Quality marker

UR - https://www.scopus.com/pages/publications/85145265137

U2 - 10.1016/j.foodchem.2022.135334

DO - 10.1016/j.foodchem.2022.135334

M3 - Journal article

C2 - 36586266

SN - 0308-8146

VL - 409

JO - Food Chemistry

JF - Food Chemistry

M1 - 135334

ER -

An oligosaccharide marker for rapid authentication of edible bird's nest

Abstract

User-Defined Keywords

UN SDGs

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