An oligosaccharide-marker approach to quantify specific polysaccharides in herbal formula by LC-qTOF-MS: Danggui Buxue Tang, a case study

Li Feng Li, Tin Long Wong, Ji Xia Zhang, Li Shuang Zhou, Su Ping Bai, Hau Yee Fung, Hui Yuan Cheng, Quan Wei Zhang, Hong Ming Zheng, Wan Rong Bao, Edmond Dik Lung MA, Chung Hang Leung, Ge ZHANG, Zhaoxiang BIAN, Aiping LYU, Simon Q B HAN*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Polysaccharides have broad bioactivities and are major components of water decoction of herb formulae. However, the quality control of polysaccharides remains a challenge. Oligosaccharide-fragment approach has been considered in elucidating chemical structures of polysaccharides, but never been used for quantitation. Using reference chemicals and a real sample Danggui Buxue Tang (DBT) in this study, an oligosaccharide-marker approach was established to quantify specific polysaccharides. Firstly, linear relationships between parent polysaccharides and hydrolysis-produced daughter oligosaccharides were verified using reference polysaccharides. Then in case of DBT, two fluorescence-labeled oligosaccharides with high specificity to individual parent polysaccharides were selected as markers. They were easily isolated and identified. Their potential in quantification of parent polysaccharides were satisfactorily validated in terms of linearity (r≥0.99), repeatability (RSD ≤ 8.4 %), and spike recovery (≥80 %). This method could be a promising approach for quality assessment of polysaccharides in herbal formulae.

Original languageEnglish
Article number113235
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume185
DOIs
Publication statusPublished - 5 Jun 2020

Scopus Subject Areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

User-Defined Keywords

  • Danggui Buxue Tang
  • LC–MS
  • Oligosaccharide
  • Polysaccharide
  • Qualitative and quantitative analysis

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