An oligonucleotide-based switch-on luminescent probe for the detection of kanamycin in aqueous solution

Ka Ho Leung, Hong Zhang He, Daniel Shiu Hin Chan, Wai Chung Fu, Chung Hang Leung, Edmond Dik Lung MA*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

69 Citations (Scopus)

Abstract

We report herein the first oligonucleotide-based luminescent method for the sensitive and selective detection of kanamycin in aqueous solution. The assay utilizes a square-planar luminescent platinum(II) complex and the kanamycin aptamer. The luminescence signal of the platinum(II) complex with the oligonucleotide is weak in the absence of kanamycin. Upon the addition of kanamycin, the aptamer changes from a random-coiled structure into a specific conformation containing a hairpin region. This facilitates intercalation of the platinum(II) complex into the bound aptamer, thereby resulting in an enhanced luminescence signal of the system in response to kanamycin. The assay is sensitive and selective towards kanamycin over other antibiotics. The assay could be used to detect trace levels of kanamycin in fish extracts with a comparable sensitivity to aqueous buffer solution.

Original languageEnglish
Pages (from-to)487-492
Number of pages6
JournalSensors and Actuators, B: Chemical
Volume177
DOIs
Publication statusPublished - 2013

Scopus Subject Areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

User-Defined Keywords

  • Aptamer
  • Kanamycin
  • Luminescent
  • Platinum(II) complex
  • Switch-on probe

Fingerprint

Dive into the research topics of 'An oligonucleotide-based switch-on luminescent probe for the detection of kanamycin in aqueous solution'. Together they form a unique fingerprint.

Cite this