An iridium(III) complex/G-quadruplex ensemble for detection of ochratoxin A based on long-lifetime luminescent

Jia Tong Zhang, Tian Shu Kang, Suk Yu Wong, Ren Jun Pei*, Edmond Dik Lung MA, Chung Hang Leung

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

A G-quadruplex-based platform has been developed for the time-resolved monitoring of ochratoxin A (OTA). The simple platform displays good sensitivity for OTA with a detection limit of 40 nM via steady-state emission spectroscopy. Notably, the platform showed a detection limit of 10.8 nM via time-resolved emission spectroscopy (TRES), which is about 4 times more sensitive than steady-state mode. Moreover, the probe showed excellent selectivity for OTA over other mycotoxins. Furthermore, OTA was successfully detected in actual herbal plant extracts samples. Our platform is the first to detect OTA using TRES to distinguish between the target signals versus the auto-fluorescence of real samples. This platform shows improved detection speed, accuracy and sensitivity with simple operation, low cost, and no requirement for complicated pre-processing.

Original languageEnglish
Pages (from-to)49-55
Number of pages7
JournalAnalytical Biochemistry
Volume580
DOIs
Publication statusPublished - 1 Sep 2019

Scopus Subject Areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

User-Defined Keywords

  • G-quadruplex
  • Herbal plant extracts
  • OTA
  • TRES

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