An iridium(III) complex/G-quadruplex ensemble for detection of ochratoxin A based on long-lifetime luminescent

Jia Tong Zhang; Tian Shu Kang; Suk Yu Wong; Ren Jun Pei; Edmond Dik Lung MA; Chung Hang Leung

doi:10.1016/j.ab.2019.06.005

An iridium(III) complex/G-quadruplex ensemble for detection of ochratoxin A based on long-lifetime luminescent

Jia Tong Zhang, Tian Shu Kang, Suk Yu Wong, Ren Jun Pei^*, Edmond Dik Lung MA, Chung Hang Leung

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

13 Citations (Scopus)

Abstract

A G-quadruplex-based platform has been developed for the time-resolved monitoring of ochratoxin A (OTA). The simple platform displays good sensitivity for OTA with a detection limit of 40 nM via steady-state emission spectroscopy. Notably, the platform showed a detection limit of 10.8 nM via time-resolved emission spectroscopy (TRES), which is about 4 times more sensitive than steady-state mode. Moreover, the probe showed excellent selectivity for OTA over other mycotoxins. Furthermore, OTA was successfully detected in actual herbal plant extracts samples. Our platform is the first to detect OTA using TRES to distinguish between the target signals versus the auto-fluorescence of real samples. This platform shows improved detection speed, accuracy and sensitivity with simple operation, low cost, and no requirement for complicated pre-processing.

Original language	English
Pages (from-to)	49-55
Number of pages	7
Journal	Analytical Biochemistry
Volume	580
DOIs	https://doi.org/10.1016/j.ab.2019.06.005
Publication status	Published - 1 Sept 2019

Scopus Subject Areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

User-Defined Keywords

G-quadruplex
Herbal plant extracts
OTA
TRES

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10.1016/j.ab.2019.06.005

Cite this

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title = "An iridium(III) complex/G-quadruplex ensemble for detection of ochratoxin A based on long-lifetime luminescent",

abstract = "A G-quadruplex-based platform has been developed for the time-resolved monitoring of ochratoxin A (OTA). The simple platform displays good sensitivity for OTA with a detection limit of 40 nM via steady-state emission spectroscopy. Notably, the platform showed a detection limit of 10.8 nM via time-resolved emission spectroscopy (TRES), which is about 4 times more sensitive than steady-state mode. Moreover, the probe showed excellent selectivity for OTA over other mycotoxins. Furthermore, OTA was successfully detected in actual herbal plant extracts samples. Our platform is the first to detect OTA using TRES to distinguish between the target signals versus the auto-fluorescence of real samples. This platform shows improved detection speed, accuracy and sensitivity with simple operation, low cost, and no requirement for complicated pre-processing.",

keywords = "G-quadruplex, Herbal plant extracts, OTA, TRES",

author = "Zhang, {Jia Tong} and Kang, {Tian Shu} and Wong, {Suk Yu} and Pei, {Ren Jun} and MA, {Edmond Dik Lung} and Leung, {Chung Hang}",

note = "Funding Information: This work is supported by Hong Kong Baptist University ( FRG2/17-18/003 ), Hong Kong; the Health and Medical Research Fund ( HMRF/14150561 ), Hong Kong; National Natural Science Foundation of China ( 21575121 , 21775131 , 21628502 ), China; Hong Kong Baptist University Century Club Sponsorship Scheme 2018 , Hong Kong; Interdisciplinary Research Matching Scheme ( RC-IRMS/16-17/03 ), Hong Kong; Interdisciplinary Research Clusters Matching Scheme ( RC-IRCs/17-18/03 ), Hong Kong; Matching Proof of Concept Fund ( MPCF-001-2017/18) , Hong Kong; Collaborative Research Fund ( C5026-16G ), Hong Kong; SKLEBA and HKBU Strategic Development Fund ( SKLP_1718_P04 ), Hong Kong; the Science and Technology Development Fund , Macao SAR ( 077/2016/A2 ), Macao; the University of Macau ( MYRG2018-00187-ICMS , MYRG2016-00151-ICMS-QRCM ), Macao. ",

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AU - Pei, Ren Jun

AU - MA, Edmond Dik Lung

AU - Leung, Chung Hang

N1 - Funding Information: This work is supported by Hong Kong Baptist University ( FRG2/17-18/003 ), Hong Kong; the Health and Medical Research Fund ( HMRF/14150561 ), Hong Kong; National Natural Science Foundation of China ( 21575121 , 21775131 , 21628502 ), China; Hong Kong Baptist University Century Club Sponsorship Scheme 2018 , Hong Kong; Interdisciplinary Research Matching Scheme ( RC-IRMS/16-17/03 ), Hong Kong; Interdisciplinary Research Clusters Matching Scheme ( RC-IRCs/17-18/03 ), Hong Kong; Matching Proof of Concept Fund ( MPCF-001-2017/18) , Hong Kong; Collaborative Research Fund ( C5026-16G ), Hong Kong; SKLEBA and HKBU Strategic Development Fund ( SKLP_1718_P04 ), Hong Kong; the Science and Technology Development Fund , Macao SAR ( 077/2016/A2 ), Macao; the University of Macau ( MYRG2018-00187-ICMS , MYRG2016-00151-ICMS-QRCM ), Macao.

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AB - A G-quadruplex-based platform has been developed for the time-resolved monitoring of ochratoxin A (OTA). The simple platform displays good sensitivity for OTA with a detection limit of 40 nM via steady-state emission spectroscopy. Notably, the platform showed a detection limit of 10.8 nM via time-resolved emission spectroscopy (TRES), which is about 4 times more sensitive than steady-state mode. Moreover, the probe showed excellent selectivity for OTA over other mycotoxins. Furthermore, OTA was successfully detected in actual herbal plant extracts samples. Our platform is the first to detect OTA using TRES to distinguish between the target signals versus the auto-fluorescence of real samples. This platform shows improved detection speed, accuracy and sensitivity with simple operation, low cost, and no requirement for complicated pre-processing.

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An iridium(III) complex/G-quadruplex ensemble for detection of ochratoxin A based on long-lifetime luminescent

Abstract

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