An iridium(III) complex-based chemosensor for the detection of thiourea in living cells

Wanhe Wang; Zhen Zhen Dong; Chao Yang; Guodong Li; Yam Chi Tse; Chung Hang Leung; Edmond Dik Lung Ma

doi:10.1016/j.snb.2017.05.100

An iridium(III) complex-based chemosensor for the detection of thiourea in living cells

Wanhe Wang
, Zhen Zhen Dong
, Chao Yang
, Guodong Li
, Yam Chi Tse
, Chung Hang Leung^*
, Edmond Dik Lung Ma^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

33 Citations (Scopus)

Abstract

The adverse effects that thiourea can induce in biological systems demand the development of new methods for its detection. In this work, we present a iridium(III) complex-based chemosensor (1) containing a recognition unit for thiourea. The reaction of the recognition unit with thiourea affects the long-lived excited states of the complex, leading to an enhancement of luminescence. Complex 1 showed a 3.3-fold enhanced luminescence in the presence of thiorea and a good linear relationship with thiourea ranging from 10 to 200 μM, with a limit of detection (LOD) of 3.85 μM. By using time-resolved emission spectroscopy (TRES), complex 1 could detect thiourea in the presence of interfering fluorescence. Moreover, complex 1 offers the capability to image thiourea in living HeLa cells. To our knowledge, complex 1 is the first iridium(III) complex-based sensor reported for imaging thiourea in living cells.

Original language	English
Pages (from-to)	374-379
Number of pages	6
Journal	Sensors and Actuators B: Chemical
Volume	251
DOIs	https://doi.org/10.1016/j.snb.2017.05.100
Publication status	Published - Nov 2017

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

User-Defined Keywords

Chemosensor
Iridium(III) complex
Luminescence
Thiourea

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10.1016/j.snb.2017.05.100

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title = "An iridium(III) complex-based chemosensor for the detection of thiourea in living cells",

abstract = "The adverse effects that thiourea can induce in biological systems demand the development of new methods for its detection. In this work, we present a iridium(III) complex-based chemosensor (1) containing a recognition unit for thiourea. The reaction of the recognition unit with thiourea affects the long-lived excited states of the complex, leading to an enhancement of luminescence. Complex 1 showed a 3.3-fold enhanced luminescence in the presence of thiorea and a good linear relationship with thiourea ranging from 10 to 200 μM, with a limit of detection (LOD) of 3.85 μM. By using time-resolved emission spectroscopy (TRES), complex 1 could detect thiourea in the presence of interfering fluorescence. Moreover, complex 1 offers the capability to image thiourea in living HeLa cells. To our knowledge, complex 1 is the first iridium(III) complex-based sensor reported for imaging thiourea in living cells.",

keywords = "Chemosensor, Iridium(III) complex, Luminescence, Thiourea",

author = "Wanhe Wang and Dong, \{Zhen Zhen\} and Chao Yang and Guodong Li and Tse, \{Yam Chi\} and Leung, \{Chung Hang\} and Ma, \{Edmond Dik Lung\}",

note = "This work is supported by Hong Kong Baptist University (FRG2/15-16/002), the Health and Medical Research fund (HMRF/14130522), the Research Grants Council (HKBU/12301115, HKBU/204612 and HKBU/201913), the National Natural Science foundation of China (21575121), the Guangdong Province Natural Science Foundation (2015A030313816), the Hong Kong Baptist University Century Club Sponsorship Scheme 2016, the Interdisciplinary Research Matching Scheme (RC-IRMS/15-16/03), the Science and Technology Development Fund, Macao SAR (098/2014/A2), and the University of Macau (MYRG2015-00137-ICMS-QRCM, MYRG2016-00151 -ICMS-QRCM and MRG044/LCH/2015/ICMS), and National Natural Science Foundation of China (21628502). ",

year = "2017",

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doi = "10.1016/j.snb.2017.05.100",

language = "English",

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pages = "374--379",

journal = "Sensors and Actuators B: Chemical",

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AU - Wang, Wanhe

AU - Dong, Zhen Zhen

AU - Yang, Chao

AU - Li, Guodong

AU - Tse, Yam Chi

AU - Leung, Chung Hang

AU - Ma, Edmond Dik Lung

N1 - This work is supported by Hong Kong Baptist University (FRG2/15-16/002), the Health and Medical Research fund (HMRF/14130522), the Research Grants Council (HKBU/12301115, HKBU/204612 and HKBU/201913), the National Natural Science foundation of China (21575121), the Guangdong Province Natural Science Foundation (2015A030313816), the Hong Kong Baptist University Century Club Sponsorship Scheme 2016, the Interdisciplinary Research Matching Scheme (RC-IRMS/15-16/03), the Science and Technology Development Fund, Macao SAR (098/2014/A2), and the University of Macau (MYRG2015-00137-ICMS-QRCM, MYRG2016-00151 -ICMS-QRCM and MRG044/LCH/2015/ICMS), and National Natural Science Foundation of China (21628502).

PY - 2017/11

Y1 - 2017/11

N2 - The adverse effects that thiourea can induce in biological systems demand the development of new methods for its detection. In this work, we present a iridium(III) complex-based chemosensor (1) containing a recognition unit for thiourea. The reaction of the recognition unit with thiourea affects the long-lived excited states of the complex, leading to an enhancement of luminescence. Complex 1 showed a 3.3-fold enhanced luminescence in the presence of thiorea and a good linear relationship with thiourea ranging from 10 to 200 μM, with a limit of detection (LOD) of 3.85 μM. By using time-resolved emission spectroscopy (TRES), complex 1 could detect thiourea in the presence of interfering fluorescence. Moreover, complex 1 offers the capability to image thiourea in living HeLa cells. To our knowledge, complex 1 is the first iridium(III) complex-based sensor reported for imaging thiourea in living cells.

AB - The adverse effects that thiourea can induce in biological systems demand the development of new methods for its detection. In this work, we present a iridium(III) complex-based chemosensor (1) containing a recognition unit for thiourea. The reaction of the recognition unit with thiourea affects the long-lived excited states of the complex, leading to an enhancement of luminescence. Complex 1 showed a 3.3-fold enhanced luminescence in the presence of thiorea and a good linear relationship with thiourea ranging from 10 to 200 μM, with a limit of detection (LOD) of 3.85 μM. By using time-resolved emission spectroscopy (TRES), complex 1 could detect thiourea in the presence of interfering fluorescence. Moreover, complex 1 offers the capability to image thiourea in living HeLa cells. To our knowledge, complex 1 is the first iridium(III) complex-based sensor reported for imaging thiourea in living cells.

KW - Chemosensor

KW - Iridium(III) complex

KW - Luminescence

KW - Thiourea

UR - https://www.scopus.com/pages/publications/85019962700

U2 - 10.1016/j.snb.2017.05.100

DO - 10.1016/j.snb.2017.05.100

M3 - Journal article

AN - SCOPUS:85019962700

SN - 0925-4005

VL - 251

SP - 374

EP - 379

JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

ER -

An iridium(III) complex-based chemosensor for the detection of thiourea in living cells

Abstract

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