An interesting two-phase solvent system and its use in preparative isolation of aconitines from aconite roots by counter-current chromatography

Simon Q B HAN*, Wai Lun Tang, Cai Xia Dong, Hong Xi Xu, Zhi Hong JIANG

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Two-phase solvent system plays crucial role in successful separation of organic compounds using counter-current chromatography (CCC). An interesting two-phase solvent system, composed of chloroform/ethyl acetate/methanol/water, is reported here, in which both phases contain sufficient organic solvents to balance their dissolving capacities. Adjusting the solvent system to get satisfactory partition coefficients (K values) for target compounds becomes relatively simple. This solvent system succeeded in sample preparation of aconitine (8.07 mg, 93.69%), hypaconitine (7.74 mg, 93.17%), mesaconitine (1.95 mg, 94.52%) from raw aconite roots (102.24 mg, crude extract), benzoylmesaconine (34.79 mg, 98.67%) from processed aconite roots (400.01 mg, crude extract), and yunaconitine (253.59 mg, 98.65%) from a crude extract of Aconitum forrestii (326.69 mg, crude extract).

Original languageEnglish
Pages (from-to)1304-1310
Number of pages7
JournalJournal of Separation Science
Volume36
Issue number7
DOIs
Publication statusPublished - Apr 2013

Scopus Subject Areas

  • Analytical Chemistry
  • Filtration and Separation

User-Defined Keywords

  • Aconitine
  • Counter-current chromatography
  • Two-phase solvent system

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