TY - JOUR
T1 - An ethanol extract of the rhizome of Atractylodes chinensis exerts anti-gastritis activities and inhibits Akt/NF-κB signaling
AU - Hossen, Muhammad Jahangir
AU - Chou, Ji Yao
AU - Li, Su Mei
AU - Fu, Xiuqiong
AU - Yin, Chengle
AU - Guo, Hui
AU - Amin, Aftab
AU - Chou, Gui Xin
AU - Yu, Zhiling
N1 - Funding Information:
This work was conducted by grants GRF: 12125116; National Natural Science Foundation of China: 81673649; STICS: JCYJ20150630164505508 and JCYJ20160229210327924; NSFG: 2016A030313007; and Hong Kong Baptist University: FRG1/16–17/048 and FRG2/16–17/033.
Publisher copyright:
© 2018 Elsevier B.V.
PY - 2019/1/10
Y1 - 2019/1/10
N2 - Ethnopharmacological relevance: The rhizome of Atractylodes chinensis (DC.) kodiz (Compositae) has traditionally been used to treat inflammatory disorders such as arthritis and stomach ache, but scanted report has been issued on its anti-inflammatory mechanisms. Aim of the study: Here, we investigated the anti-gastritis activities and explored the mechanism of action of an ethanolic extract of the herb (Ac-EE). Materials and methods: Ac-EE was prepared with 95% ethanol. To determine its in vivo effects, we employed an HCl/EtOH-induced gastritis rat model. We used a lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage model for in vitro assays. Griess and MTT assays were used to measure nitric oxide (NO) production and cell viability, respectively. We used real-time PCR to determine mRNA levels. To measure prostaglandin E2 (PGE2) production we used a PGE2 EIA kit. To estimate protein levels and enzyme activities, we employed immunoblotting. Luciferase assays were used to examine nuclear transcription factor (NF)-κB activities. Results: Intragastric administration of Ac-EE (30 mg/kg) ameliorated HCl/EtOH-induced stomach tissue damages in SD rats. Ac-EE inhibited the levels of NO and PGE2 down regulated mRNA and protein levels of inducible NO synthase (iNOS) and cyclooxygenase (COX)−2. Ac-EE suppressed the nuclear level of NF-κB (p50), and inhibited NF-κB luciferase activity. The Phosphorylation of Akt and IκBα was also inhibited by Ac-EE both in vivo and in vitro. Conclusion: Ac-EE treatment exerts an anti-gastritis effect in rats. Inhibition of the Akt/IκBα/NF-κB signaling pathway is associated with this effect, providing a pharmacological basis for the clinical application of the rhizome of A. chinensis in the treatment of inflammatory diseases.
AB - Ethnopharmacological relevance: The rhizome of Atractylodes chinensis (DC.) kodiz (Compositae) has traditionally been used to treat inflammatory disorders such as arthritis and stomach ache, but scanted report has been issued on its anti-inflammatory mechanisms. Aim of the study: Here, we investigated the anti-gastritis activities and explored the mechanism of action of an ethanolic extract of the herb (Ac-EE). Materials and methods: Ac-EE was prepared with 95% ethanol. To determine its in vivo effects, we employed an HCl/EtOH-induced gastritis rat model. We used a lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage model for in vitro assays. Griess and MTT assays were used to measure nitric oxide (NO) production and cell viability, respectively. We used real-time PCR to determine mRNA levels. To measure prostaglandin E2 (PGE2) production we used a PGE2 EIA kit. To estimate protein levels and enzyme activities, we employed immunoblotting. Luciferase assays were used to examine nuclear transcription factor (NF)-κB activities. Results: Intragastric administration of Ac-EE (30 mg/kg) ameliorated HCl/EtOH-induced stomach tissue damages in SD rats. Ac-EE inhibited the levels of NO and PGE2 down regulated mRNA and protein levels of inducible NO synthase (iNOS) and cyclooxygenase (COX)−2. Ac-EE suppressed the nuclear level of NF-κB (p50), and inhibited NF-κB luciferase activity. The Phosphorylation of Akt and IκBα was also inhibited by Ac-EE both in vivo and in vitro. Conclusion: Ac-EE treatment exerts an anti-gastritis effect in rats. Inhibition of the Akt/IκBα/NF-κB signaling pathway is associated with this effect, providing a pharmacological basis for the clinical application of the rhizome of A. chinensis in the treatment of inflammatory diseases.
KW - Akt
KW - Atractylenolide I (PubChem ID: 6321018)
KW - Atractylenolide II (PubChem ID: 14448070)
KW - Atractylodes chinensis
KW - Gastritis
KW - Lipopolysaccharide (PubChem ID: 11970143)
KW - NF-κB
KW - NO
KW - PGE
UR - http://www.scopus.com/inward/record.url?scp=85053766199&partnerID=8YFLogxK
U2 - 10.1016/j.jep.2018.09.015
DO - 10.1016/j.jep.2018.09.015
M3 - Journal article
C2 - 30218812
AN - SCOPUS:85053766199
SN - 0378-8741
VL - 228
SP - 18
EP - 25
JO - Journal of Ethnopharmacology
JF - Journal of Ethnopharmacology
ER -
