An ethanol biosensor based on a bacterial cell-immobilized eggshell membrane

Guang Ming Wen, Shao Min Shuang, Chuan Dong*, Martin M F CHOI

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

11 Citations (Scopus)


An ethanol biosensor was fabricated based on a Methylobacterium organophilium-immobilized eggshell membrane and an oxygen (O2) electrode. A linear response for ethanol was obtained in the range of 0.050-7.5 mmol/L with a detection limit of 0.025 mmol/L (S/N = 3) and a R.S.D. of 2.1%. The response time was less than 100 s at room temperature and ambient pressure. The optimal loading of bacterial cells on the biosensor membrane is 40 mg (wet weight). The optimal working conditions for the microbial biosensor are pH 7.0 phosphate buffer (50 mmol/L) at 20-25°C. The interference test, operational and storage stability of the biosensor are studied in detail. Finally, the biosensor is applied to determine the ethanol contents in various alcohol samples and the results are comparable to that obtained by gas chromatographic method and the results are satisfactory. Our proposed biosensor provides a convenient, simple and reliable method to determine ethanol content in alcoholic drinks.

Original languageEnglish
Pages (from-to)481-483
Number of pages3
JournalChinese Chemical Letters
Issue number4
Publication statusPublished - Apr 2012

Scopus Subject Areas

  • Chemistry(all)

User-Defined Keywords

  • Eggshell membrane
  • Ethanol
  • Methylobacterium organophilium
  • Microbial biosensor
  • Oxygen electrode
  • Wine


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