An Essential and Cell-Cycle-Dependent ORC Dimerization Cycle Regulates Eukaryotic Chromosomal DNA Replication

Aftab Amin; Rentian Wu; Man Hei Cheung; John F. Scott; Ziyi Wang; Zijing Zhou; Changdong Liu; Guang Zhu; Chris Kong Chu Wong; Zhiling Yu; Chun Liang

doi:10.1016/j.celrep.2020.02.046

An Essential and Cell-Cycle-Dependent ORC Dimerization Cycle Regulates Eukaryotic Chromosomal DNA Replication

Aftab Amin
, Rentian Wu
, Man Hei Cheung
, John F. Scott
, Ziyi Wang
, Zijing Zhou
, Changdong Liu
, Guang Zhu
, Chris Kong Chu Wong
, Zhiling Yu^*
, Chun Liang^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

12 Citations (Scopus)

Abstract

Amin et al. provide in vivo evidence for an essential, cell-cycle-dependent, and likely semi-conservative ORC dimerization cycle that regulates eukaryotic chromosomal DNA replication. ORC dimers provide symmetric platforms to load the symmetric pre-RCs at replication origins. ORC de-dimerization produces two single hexamers to bind and protect the two nascent origins.

Original language	English
Pages (from-to)	3323-3338.e6
Number of pages	23
Journal	Cell Reports
Volume	30
Issue number	10
DOIs	https://doi.org/10.1016/j.celrep.2020.02.046
Publication status	Published - 10 Mar 2020

User-Defined Keywords

cell-cycle control
DNA replication
ORC dimerization
pre-RC formation
replication licensing

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10.1016/j.celrep.2020.02.046

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title = "An Essential and Cell-Cycle-Dependent ORC Dimerization Cycle Regulates Eukaryotic Chromosomal DNA Replication",

abstract = "Amin et al. provide in vivo evidence for an essential, cell-cycle-dependent, and likely semi-conservative ORC dimerization cycle that regulates eukaryotic chromosomal DNA replication. ORC dimers provide symmetric platforms to load the symmetric pre-RCs at replication origins. ORC de-dimerization produces two single hexamers to bind and protect the two nascent origins.",

keywords = "cell-cycle control, DNA replication, ORC dimerization, pre-RC formation, replication licensing",

author = "Aftab Amin and Rentian Wu and Cheung, \{Man Hei\} and Scott, \{John F.\} and Ziyi Wang and Zijing Zhou and Changdong Liu and Guang Zhu and Wong, \{Chris Kong Chu\} and Zhiling Yu and Chun Liang",

note = "Funding Information: We thank Bruce Stillman and Ulrich Laemmli for the kind gifts of yeast strains, plasmids, and/or antibodies and Yuanliang Zhai, Chuchu Zhang, Jiafeng Wang, Langhuan Pei, and Kelvin K.L. Sou for technical assistance. This work was supported by the Hong Kong Research Grants Council (GRF661713 and RGC No. 16103717), the Science and Technology Innovation Bureau of Guangzhou Development District (CY2019-005), and Hong Kong Baptist University, Research Committee, Initiation Grant - Faculty Niche Research Areas (IG-FNRA) 2018/19 (RC-FNRA-IG-18-19-SCM-01). Publisher copyright: {\textcopyright} 2020 The Author(s).",

year = "2020",

month = mar,

day = "10",

doi = "10.1016/j.celrep.2020.02.046",

language = "English",

volume = "30",

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journal = "Cell Reports",

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T1 - An Essential and Cell-Cycle-Dependent ORC Dimerization Cycle Regulates Eukaryotic Chromosomal DNA Replication

AU - Amin, Aftab

AU - Wu, Rentian

AU - Cheung, Man Hei

AU - Scott, John F.

AU - Wang, Ziyi

AU - Zhou, Zijing

AU - Liu, Changdong

AU - Zhu, Guang

AU - Wong, Chris Kong Chu

AU - Yu, Zhiling

AU - Liang, Chun

N1 - Funding Information: We thank Bruce Stillman and Ulrich Laemmli for the kind gifts of yeast strains, plasmids, and/or antibodies and Yuanliang Zhai, Chuchu Zhang, Jiafeng Wang, Langhuan Pei, and Kelvin K.L. Sou for technical assistance. This work was supported by the Hong Kong Research Grants Council (GRF661713 and RGC No. 16103717), the Science and Technology Innovation Bureau of Guangzhou Development District (CY2019-005), and Hong Kong Baptist University, Research Committee, Initiation Grant - Faculty Niche Research Areas (IG-FNRA) 2018/19 (RC-FNRA-IG-18-19-SCM-01). Publisher copyright: © 2020 The Author(s).

PY - 2020/3/10

Y1 - 2020/3/10

N2 - Amin et al. provide in vivo evidence for an essential, cell-cycle-dependent, and likely semi-conservative ORC dimerization cycle that regulates eukaryotic chromosomal DNA replication. ORC dimers provide symmetric platforms to load the symmetric pre-RCs at replication origins. ORC de-dimerization produces two single hexamers to bind and protect the two nascent origins.

AB - Amin et al. provide in vivo evidence for an essential, cell-cycle-dependent, and likely semi-conservative ORC dimerization cycle that regulates eukaryotic chromosomal DNA replication. ORC dimers provide symmetric platforms to load the symmetric pre-RCs at replication origins. ORC de-dimerization produces two single hexamers to bind and protect the two nascent origins.

KW - cell-cycle control

KW - DNA replication

KW - ORC dimerization

KW - pre-RC formation

KW - replication licensing

UR - https://www.scopus.com/pages/publications/85080985562

U2 - 10.1016/j.celrep.2020.02.046

DO - 10.1016/j.celrep.2020.02.046

M3 - Journal article

C2 - 32160540

AN - SCOPUS:85080985562

SN - 2211-1247

VL - 30

SP - 3323-3338.e6

JO - Cell Reports

JF - Cell Reports

IS - 10

ER -

An Essential and Cell-Cycle-Dependent ORC Dimerization Cycle Regulates Eukaryotic Chromosomal DNA Replication

Abstract

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