An Essential and Cell-Cycle-Dependent ORC Dimerization Cycle Regulates Eukaryotic Chromosomal DNA Replication

Aftab Amin, Rentian Wu, Man Hei Cheung, John F. Scott, Ziyi Wang, Zijing Zhou, Changdong Liu, Guang Zhu, Chris Kong Chu Wong, Zhiling Yu*, Chun Liang*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

8 Citations (Scopus)

Abstract

Amin et al. provide in vivo evidence for an essential, cell-cycle-dependent, and likely semi-conservative ORC dimerization cycle that regulates eukaryotic chromosomal DNA replication. ORC dimers provide symmetric platforms to load the symmetric pre-RCs at replication origins. ORC de-dimerization produces two single hexamers to bind and protect the two nascent origins.

Original languageEnglish
Pages (from-to)3323-3338.e6
Number of pages23
JournalCell Reports
Volume30
Issue number10
DOIs
Publication statusPublished - 10 Mar 2020

Scopus Subject Areas

  • General Biochemistry,Genetics and Molecular Biology

User-Defined Keywords

  • cell-cycle control
  • DNA replication
  • ORC dimerization
  • pre-RC formation
  • replication licensing

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