TY - JOUR
T1 - An end-channel amperometric detector for microchip capillary electrophoresis
AU - Wu, Youyi
AU - Lin, Jin Ming
AU - Su, Rongguo
AU - Qu, Feng
AU - CAI, Zongwei
N1 - Funding Information:
This work was supported by National Science Fund for Distinguished Youth Scholars of China (20125514), National Natural Science Foundation of China (50273046, 20377046) and 863 Program (2001AA635030).
PY - 2004/10/8
Y1 - 2004/10/8
N2 - An end-channel amperometric detector with a guide tube for working electrode was designed and integrated on a home-made glass microchip. The guide tube was directly patterned and fabricated at the end of the detection reservoir, which made the fixation and alignment of working electrode relatively easy. The fabrication was carried out in a two-step etching process. A 30 μm carbon fiber microdisk electrode and Pt cathode were also integrated onto the amperometric detector. The characteristics and primary performance of the home-made microchip capillary electrophoresis (MCCE) were investigated with neurotransmitters. The baseline separation of dopamine (DA), catechol (CA) and epinephrine (EP) was achieved within 80 s. Separation parameters such as injection time, buffer components, pH of the buffer were studied. Relative standard deviations of not more than 6.0% were obtained for both peak currents and migration times. Under the selected separation conditions, the response for DA was linear from 5 to 200 μM and from 20 to 800 μM for CA. The limits of detection of DA and CA were 0.51 and 2.9 μM, respectively (S/N=3).
AB - An end-channel amperometric detector with a guide tube for working electrode was designed and integrated on a home-made glass microchip. The guide tube was directly patterned and fabricated at the end of the detection reservoir, which made the fixation and alignment of working electrode relatively easy. The fabrication was carried out in a two-step etching process. A 30 μm carbon fiber microdisk electrode and Pt cathode were also integrated onto the amperometric detector. The characteristics and primary performance of the home-made microchip capillary electrophoresis (MCCE) were investigated with neurotransmitters. The baseline separation of dopamine (DA), catechol (CA) and epinephrine (EP) was achieved within 80 s. Separation parameters such as injection time, buffer components, pH of the buffer were studied. Relative standard deviations of not more than 6.0% were obtained for both peak currents and migration times. Under the selected separation conditions, the response for DA was linear from 5 to 200 μM and from 20 to 800 μM for CA. The limits of detection of DA and CA were 0.51 and 2.9 μM, respectively (S/N=3).
KW - Carbon fiber microdisk electrode
KW - End-channel amperometric detection
KW - Guide tube
KW - Microchip capillary electrophoresis
KW - Two-step etching
UR - http://www.scopus.com/inward/record.url?scp=4143137851&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2004.02.033
DO - 10.1016/j.talanta.2004.02.033
M3 - Article
AN - SCOPUS:4143137851
SN - 0039-9140
VL - 64
SP - 338
EP - 344
JO - Talanta
JF - Talanta
IS - 2
ER -