TY - JOUR
T1 - Amyloplast is involved in the MIZ1-modulated root hydrotropism
AU - Hong, Yonghui
AU - Liu, Siqi
AU - Chen, Yadi
AU - Yao, Zixuan
AU - Jiang, Shuqiu
AU - Wang, Lulu
AU - Zhu, Xinkai
AU - Xu, Weifeng
AU - Zhang, Jianhua
AU - Li, Ying
N1 - Funding information:
This research is funded by the Natural Science Foundation of Jiangsu Province (BK20220566), the National Natural Science Foundation of China (32301761), the Hong Kong Scholars Program (XJ2023052), the China Postdoctoral Science Foundation (2023M733003) and the College Student Science and Technology Innovation Foundation of Yangzhou University (XCX20230647). We are also grateful for a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
Publisher Copyright:
© 2024 Elsevier GmbH
PY - 2024/5
Y1 - 2024/5
N2 - Roots exhibit hydrotropism in response to moisture gradients, with the hydrotropism-related gene Mizu-kussei1 (MIZ1) playing a role in regulating root hydrotropism in an oblique orientation. However, the mechanisms underlying MIZ1-regulated root hydrotropism are not well understood. In this study, we employed obliquely oriented experimental systems to investigate root hydrotropism in Arabidopsis. We found that the miz1 mutant displays reduced root hydrotropism but increased root gravitropism following hydrostimulation, as compared to wild-type plants. Conversely, overexpression of AtMIZ1 leads to enhanced root hydrotropism but decreased root gravitropism following hydrostimulation, as compared to wild-type plants. Using co-immunoprecipitation followed by mass spectrometry (IP-MS), we explored proteins that interact with AtMIZ1, and we identified PGMC1 co-immunoprecipitated with MIZ1 in vivo. Furthermore, the miz1 mutant exhibited higher expression of the PGMC1 gene and increased phosphoglucomutase (PGM) activity, while AtMIZ1 overexpressors resulted in lower expression of the PGMC1 gene, reduced amyloplast amount, and reduced PGM activity in comparison to wild-type roots. In addition, different Arabidopsis natural accessions having difference in their hydrotropic response demonstrated expression level of PGMC1 was negatively correlated with hydrotropic root curvature and AtMIZ1 expression. Our results provide valuable insights into the role of amyloplast in MIZ1-regulated root hydrotropism.
AB - Roots exhibit hydrotropism in response to moisture gradients, with the hydrotropism-related gene Mizu-kussei1 (MIZ1) playing a role in regulating root hydrotropism in an oblique orientation. However, the mechanisms underlying MIZ1-regulated root hydrotropism are not well understood. In this study, we employed obliquely oriented experimental systems to investigate root hydrotropism in Arabidopsis. We found that the miz1 mutant displays reduced root hydrotropism but increased root gravitropism following hydrostimulation, as compared to wild-type plants. Conversely, overexpression of AtMIZ1 leads to enhanced root hydrotropism but decreased root gravitropism following hydrostimulation, as compared to wild-type plants. Using co-immunoprecipitation followed by mass spectrometry (IP-MS), we explored proteins that interact with AtMIZ1, and we identified PGMC1 co-immunoprecipitated with MIZ1 in vivo. Furthermore, the miz1 mutant exhibited higher expression of the PGMC1 gene and increased phosphoglucomutase (PGM) activity, while AtMIZ1 overexpressors resulted in lower expression of the PGMC1 gene, reduced amyloplast amount, and reduced PGM activity in comparison to wild-type roots. In addition, different Arabidopsis natural accessions having difference in their hydrotropic response demonstrated expression level of PGMC1 was negatively correlated with hydrotropic root curvature and AtMIZ1 expression. Our results provide valuable insights into the role of amyloplast in MIZ1-regulated root hydrotropism.
KW - Amyloplast
KW - Gravitropism
KW - Hydrotropism
KW - MIZ1
KW - Root
UR - http://www.scopus.com/inward/record.url?scp=85188102234&partnerID=8YFLogxK
U2 - 10.1016/j.jplph.2024.154224
DO - 10.1016/j.jplph.2024.154224
M3 - Journal article
AN - SCOPUS:85188102234
SN - 0176-1617
VL - 296
JO - Journal of Plant Physiology
JF - Journal of Plant Physiology
M1 - 154224
ER -