Amyloplast is involved in the MIZ1-modulated root hydrotropism

Yonghui Hong, Siqi Liu, Yadi Chen, Zixuan Yao, Shuqiu Jiang, Lulu Wang, Xinkai Zhu, Weifeng Xu, Jianhua Zhang*, Ying Li*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

Abstract

Roots exhibit hydrotropism in response to moisture gradients, with the hydrotropism-related gene Mizu-kussei1 (MIZ1) playing a role in regulating root hydrotropism in an oblique orientation. However, the mechanisms underlying MIZ1-regulated root hydrotropism are not well understood. In this study, we employed obliquely oriented experimental systems to investigate root hydrotropism in Arabidopsis. We found that the miz1 mutant displays reduced root hydrotropism but increased root gravitropism following hydrostimulation, as compared to wild-type plants. Conversely, overexpression of AtMIZ1 leads to enhanced root hydrotropism but decreased root gravitropism following hydrostimulation, as compared to wild-type plants. Using co-immunoprecipitation followed by mass spectrometry (IP-MS), we explored proteins that interact with AtMIZ1, and we identified PGMC1 co-immunoprecipitated with MIZ1 in vivo. Furthermore, the miz1 mutant exhibited higher expression of the PGMC1 gene and increased phosphoglucomutase (PGM) activity, while AtMIZ1 overexpressors resulted in lower expression of the PGMC1 gene, reduced amyloplast amount, and reduced PGM activity in comparison to wild-type roots. In addition, different Arabidopsis natural accessions having difference in their hydrotropic response demonstrated expression level of PGMC1 was negatively correlated with hydrotropic root curvature and AtMIZ1 expression. Our results provide valuable insights into the role of amyloplast in MIZ1-regulated root hydrotropism.

Original languageEnglish
Article number154224
Number of pages6
JournalJournal of Plant Physiology
Volume296
Early online date15 Mar 2024
DOIs
Publication statusPublished - May 2024

Scopus Subject Areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

User-Defined Keywords

  • Amyloplast
  • Gravitropism
  • Hydrotropism
  • MIZ1
  • Root

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