TY - JOUR
T1 - Alterations in acetylcholinesterase and choline acetyltransferase activities and neuropeptide levels in the ventral spinal cord of the Wobbler mouse during inherited motoneuron disease
AU - Yung, Kin Lam
AU - Tang, F
AU - Vacca-Gallowaya, L.L.
N1 - This research is supported by the following grants: UPGC (338/031/0006), Croucher Foundation (360/031/0814), Run Run Shaw Foundation (372/162/6396), and CRCG (372/162/6994).
PY - 1994/2/28
Y1 - 1994/2/28
N2 - Enymatic assays for acetylcholine esterase (AChE) and choline acetyltransferase (ChAT) were applied to dorsal and ventral cervical spinal cord regions taken from the Wobbler mouse, a model for inherited motoneuron disease. Early in the disease, ChAT (but not AChE) activity is significantly greater compared with the control littermate specimens. The high ChAT activity correlates with the high thyrotopin releasing hormone (also leucine-enkephalin) concentrations measured in the Wobbler ventral horn early in the disease. Late in the motoneuron disease, both AChE and ChAT activities are significantly lower than in the control littermate spicemens. These data correlate with the high substance P, methionine and leucine enkephalin concentrations measured in the Wobbler ventral horn late in the motoneuron disease.
AB - Enymatic assays for acetylcholine esterase (AChE) and choline acetyltransferase (ChAT) were applied to dorsal and ventral cervical spinal cord regions taken from the Wobbler mouse, a model for inherited motoneuron disease. Early in the disease, ChAT (but not AChE) activity is significantly greater compared with the control littermate specimens. The high ChAT activity correlates with the high thyrotopin releasing hormone (also leucine-enkephalin) concentrations measured in the Wobbler ventral horn early in the disease. Late in the motoneuron disease, both AChE and ChAT activities are significantly lower than in the control littermate spicemens. These data correlate with the high substance P, methionine and leucine enkephalin concentrations measured in the Wobbler ventral horn late in the motoneuron disease.
KW - Radioimmunoassay
KW - Radioenzymatic assay
KW - Wobbler mouse motoneuron disease
KW - Cervical spinal cord
KW - Acetylcholinesterase
KW - Choline acetyltransferase
KW - Thyrotropin-releasing hormone
KW - Substance P
KW - Methionine-enkephalin
KW - Leucine-enkephalin
U2 - 10.1016/0006-8993(94)90668-8
DO - 10.1016/0006-8993(94)90668-8
M3 - Journal article
SN - 0006-8993
VL - 638
SP - 337
EP - 342
JO - Brain Research
JF - Brain Research
IS - 1-2
ER -