TY - JOUR
T1 - Alcohol sensing membrane based on immobilized ruthenium(II) complex in carboxylated PVC and surface covalently bonded alcohol oxidase
AU - Lau, Rebecca Choi Wan
AU - Choi, Martin Ming Fat
AU - Lu, Jianzhong
N1 - Funding Information:
The authors express their appreciation to HKUST for the use of FAB-MS, 1 H NMR and gas chromatograph. Financial support from HKBU (Project Nos. FRG/96-97/II-54 and FRG/96-97/II-62) is gratefully acknowledged.
PY - 1999/2
Y1 - 1999/2
N2 - Alcohol sensing membranes coated on overhead transparency films for the continuous monitoring of ethanol, propanol and butanol are presented. Alcohol oxidation catalyzed by alcohol oxidase in conjunction with the fluorescence quenching reaction of oxygen-sensitive dye ion-pair, tris(4,7-diphenyl-1,10- phenanthroline) ruthenium(II) didodecylsulphate was chosen for the determination. Alcohol oxidase was immobilized covalently on a plasticized carboxylated poly(vinyl chloride) membrane and the oxygen-sensitive dye ion- pair was entrapped in the same membrane. The sensing membrane relates oxygen consumption, as a result of enzymatic oxidation, to alcohol concentration. Measurements have been performed in air-saturated alcohol standard solutions of pH 7.0. Storage stability, reproducibility and the effect of pH on sensing membrane performance have been studied in detail. The alcohol sensing membrane proposed here is simple to prepare and has a fairly rapid response time of < 1 min. It has been successfully applied to the determination of the ethanol contents in various spirits.
AB - Alcohol sensing membranes coated on overhead transparency films for the continuous monitoring of ethanol, propanol and butanol are presented. Alcohol oxidation catalyzed by alcohol oxidase in conjunction with the fluorescence quenching reaction of oxygen-sensitive dye ion-pair, tris(4,7-diphenyl-1,10- phenanthroline) ruthenium(II) didodecylsulphate was chosen for the determination. Alcohol oxidase was immobilized covalently on a plasticized carboxylated poly(vinyl chloride) membrane and the oxygen-sensitive dye ion- pair was entrapped in the same membrane. The sensing membrane relates oxygen consumption, as a result of enzymatic oxidation, to alcohol concentration. Measurements have been performed in air-saturated alcohol standard solutions of pH 7.0. Storage stability, reproducibility and the effect of pH on sensing membrane performance have been studied in detail. The alcohol sensing membrane proposed here is simple to prepare and has a fairly rapid response time of < 1 min. It has been successfully applied to the determination of the ethanol contents in various spirits.
KW - Alcohol oxidation
KW - Carboxylated PVC
KW - Ruthenium(II) didodecylsulphate
UR - http://www.scopus.com/inward/record.url?scp=0032939507&partnerID=8YFLogxK
U2 - 10.1016/S0039-9140(98)00248-3
DO - 10.1016/S0039-9140(98)00248-3
M3 - Journal article
AN - SCOPUS:0032939507
SN - 0039-9140
VL - 48
SP - 321
EP - 331
JO - Talanta
JF - Talanta
IS - 2
ER -