Alcohol sensing membrane based on immobilized ruthenium(II) complex in carboxylated PVC and surface covalently bonded alcohol oxidase

Rebecca Choi Wan Lau, Martin M F CHOI*, Jianzhong Lu

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

19 Citations (Scopus)

Abstract

Alcohol sensing membranes coated on overhead transparency films for the continuous monitoring of ethanol, propanol and butanol are presented. Alcohol oxidation catalyzed by alcohol oxidase in conjunction with the fluorescence quenching reaction of oxygen-sensitive dye ion-pair, tris(4,7-diphenyl-1,10- phenanthroline) ruthenium(II) didodecylsulphate was chosen for the determination. Alcohol oxidase was immobilized covalently on a plasticized carboxylated poly(vinyl chloride) membrane and the oxygen-sensitive dye ion- pair was entrapped in the same membrane. The sensing membrane relates oxygen consumption, as a result of enzymatic oxidation, to alcohol concentration. Measurements have been performed in air-saturated alcohol standard solutions of pH 7.0. Storage stability, reproducibility and the effect of pH on sensing membrane performance have been studied in detail. The alcohol sensing membrane proposed here is simple to prepare and has a fairly rapid response time of < 1 min. It has been successfully applied to the determination of the ethanol contents in various spirits.

Original languageEnglish
Pages (from-to)321-331
Number of pages11
JournalTalanta
Volume48
Issue number2
DOIs
Publication statusPublished - Feb 1999

Scopus Subject Areas

  • Analytical Chemistry

User-Defined Keywords

  • Alcohol oxidation
  • Carboxylated PVC
  • Ruthenium(II) didodecylsulphate

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