Akt downstream of NFκB, MAPKs and IRF3 pathway involved in macrophage activation induced by Astragalus polysaccharide RAP

Zhipeng Li; Li Feng Li; Quan Wei Zhang; Wei Wei; Hong Bing Liu; Wan Rong Bao; Edmond Dik Lung Ma; Chung Hang Leung; Zhaoxiang Bian; Aiping Lyu; Simon Q B Han

doi:10.1016/j.jff.2017.10.004

Akt downstream of NFκB, MAPKs and IRF3 pathway involved in macrophage activation induced by Astragalus polysaccharide RAP

Zhipeng Li, Li Feng Li, Quan Wei Zhang, Wei Wei, Hong Bing Liu, Wan Rong Bao, Edmond Dik Lung Ma, Chung Hang Leung, Zhaoxiang Bian, Aiping Lyu^*, Simon Q B Han

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

12 Citations (Scopus)

Abstract

Polysaccharides are considered the main active ingredient for the immune-enhancing effect of Radix Astragali, which is popularly used as medicinal food supplementary. The signal pathways involved in immune-enhancing effect remain unclear. This study is to investigate the role and regulation relation of Akt pathway with downstream kinases of TLR4 receptor in macrophages induced by Astragalus polysaccharide, RAP. The results showed that RAP induced phosphorylation of Akt, NFκB, IRF3 and MAPKs in RAW264.7 cells, and their individual inhibitors not only inhibited their inflammatory factors production and gene expression, but also suppressed the Akt phosphorylation, while Akt inhibitor exerted no effect on MAPKs, NFκB, and IRF3 phosphorylation. Taken together, these results suggested that Akt and IRF3 exerted activation role in RAP induced macrophage, and Akt exerted effect downstream of MyD88 dependent and independent pathway. This study will provide a deeper insight into the signal transduction induced by Astragalus polysaccharides.

Original language	English
Pages (from-to)	152-159
Number of pages	8
Journal	Journal of Functional Foods
Volume	39
DOIs	https://doi.org/10.1016/j.jff.2017.10.004
Publication status	Published - Dec 2017

Scopus Subject Areas

Food Science
Medicine (miscellaneous)
Nutrition and Dietetics

User-Defined Keywords

Inflammatory
MyD88
Phosphorylation
Polysaccharide
RAW264.7

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10.1016/j.jff.2017.10.004

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@article{bf7bb9a96a014898a2f65e5c95628546,

title = "Akt downstream of NFκB, MAPKs and IRF3 pathway involved in macrophage activation induced by Astragalus polysaccharide RAP",

abstract = "Polysaccharides are considered the main active ingredient for the immune-enhancing effect of Radix Astragali, which is popularly used as medicinal food supplementary. The signal pathways involved in immune-enhancing effect remain unclear. This study is to investigate the role and regulation relation of Akt pathway with downstream kinases of TLR4 receptor in macrophages induced by Astragalus polysaccharide, RAP. The results showed that RAP induced phosphorylation of Akt, NFκB, IRF3 and MAPKs in RAW264.7 cells, and their individual inhibitors not only inhibited their inflammatory factors production and gene expression, but also suppressed the Akt phosphorylation, while Akt inhibitor exerted no effect on MAPKs, NFκB, and IRF3 phosphorylation. Taken together, these results suggested that Akt and IRF3 exerted activation role in RAP induced macrophage, and Akt exerted effect downstream of MyD88 dependent and independent pathway. This study will provide a deeper insight into the signal transduction induced by Astragalus polysaccharides.",

keywords = "Inflammatory, MyD88, Phosphorylation, Polysaccharide, RAW264.7",

author = "Zhipeng Li and Li, {Li Feng} and Zhang, {Quan Wei} and Wei Wei and Liu, {Hong Bing} and Bao, {Wan Rong} and Ma, {Edmond Dik Lung} and Leung, {Chung Hang} and Zhaoxiang Bian and Aiping Lyu and Han, {Simon Q B}",

note = "Funding Information: This study was supported by HKSAR Innovation and Technology Fund (ITF), Tier 3, ITS/311/09, General Research Fund ( 12100615 , 22100014 ), Health Medical Research Fund ( 11122531 , 14150521 ), National Natural Sciences Foundation in China ( 81473341 ), and Hong Kong Baptist University (RC-start up grant, MPCF-001-2014/2015, FRG1/16-17/032 and FRG2/14-15/028).",

year = "2017",

month = dec,

doi = "10.1016/j.jff.2017.10.004",

language = "English",

volume = "39",

pages = "152--159",

journal = "Journal of Functional Foods",

issn = "1756-4646",

publisher = "Elsevier Ltd.",

}

TY - JOUR

T1 - Akt downstream of NFκB, MAPKs and IRF3 pathway involved in macrophage activation induced by Astragalus polysaccharide RAP

AU - Li, Zhipeng

AU - Li, Li Feng

AU - Zhang, Quan Wei

AU - Wei, Wei

AU - Liu, Hong Bing

AU - Bao, Wan Rong

AU - Ma, Edmond Dik Lung

AU - Leung, Chung Hang

AU - Bian, Zhaoxiang

AU - Lyu, Aiping

AU - Han, Simon Q B

N1 - Funding Information: This study was supported by HKSAR Innovation and Technology Fund (ITF), Tier 3, ITS/311/09, General Research Fund ( 12100615 , 22100014 ), Health Medical Research Fund ( 11122531 , 14150521 ), National Natural Sciences Foundation in China ( 81473341 ), and Hong Kong Baptist University (RC-start up grant, MPCF-001-2014/2015, FRG1/16-17/032 and FRG2/14-15/028).

PY - 2017/12

Y1 - 2017/12

N2 - Polysaccharides are considered the main active ingredient for the immune-enhancing effect of Radix Astragali, which is popularly used as medicinal food supplementary. The signal pathways involved in immune-enhancing effect remain unclear. This study is to investigate the role and regulation relation of Akt pathway with downstream kinases of TLR4 receptor in macrophages induced by Astragalus polysaccharide, RAP. The results showed that RAP induced phosphorylation of Akt, NFκB, IRF3 and MAPKs in RAW264.7 cells, and their individual inhibitors not only inhibited their inflammatory factors production and gene expression, but also suppressed the Akt phosphorylation, while Akt inhibitor exerted no effect on MAPKs, NFκB, and IRF3 phosphorylation. Taken together, these results suggested that Akt and IRF3 exerted activation role in RAP induced macrophage, and Akt exerted effect downstream of MyD88 dependent and independent pathway. This study will provide a deeper insight into the signal transduction induced by Astragalus polysaccharides.

AB - Polysaccharides are considered the main active ingredient for the immune-enhancing effect of Radix Astragali, which is popularly used as medicinal food supplementary. The signal pathways involved in immune-enhancing effect remain unclear. This study is to investigate the role and regulation relation of Akt pathway with downstream kinases of TLR4 receptor in macrophages induced by Astragalus polysaccharide, RAP. The results showed that RAP induced phosphorylation of Akt, NFκB, IRF3 and MAPKs in RAW264.7 cells, and their individual inhibitors not only inhibited their inflammatory factors production and gene expression, but also suppressed the Akt phosphorylation, while Akt inhibitor exerted no effect on MAPKs, NFκB, and IRF3 phosphorylation. Taken together, these results suggested that Akt and IRF3 exerted activation role in RAP induced macrophage, and Akt exerted effect downstream of MyD88 dependent and independent pathway. This study will provide a deeper insight into the signal transduction induced by Astragalus polysaccharides.

KW - Inflammatory

KW - MyD88

KW - Phosphorylation

KW - Polysaccharide

KW - RAW264.7

UR - http://www.scopus.com/inward/record.url?scp=85031784609&partnerID=8YFLogxK

U2 - 10.1016/j.jff.2017.10.004

DO - 10.1016/j.jff.2017.10.004

M3 - Journal article

AN - SCOPUS:85031784609

SN - 1756-4646

VL - 39

SP - 152

EP - 159

JO - Journal of Functional Foods

JF - Journal of Functional Foods

ER -

Akt downstream of NFκB, MAPKs and IRF3 pathway involved in macrophage activation induced by Astragalus polysaccharide RAP

Abstract

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