TY - JOUR
T1 - Agrowaste to vanillin conversion by a natural Pediococcus acidilactici strain BD16
AU - Chakraborty, Debkumar
AU - Kaur, Baljinder
AU - Obulisamy, Karthikeyan
AU - Selvam, Ammaiyappan
AU - WONG, Jonathan W C
N1 - Publisher Copyright:
© 2016 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2017/7/18
Y1 - 2017/7/18
N2 - Owing to its flavoring, antimicrobial, antioxidant and anticarcinogenic nature, vanillin is widely used in foods, beverages, perfumes and pharmaceutical products. Ferulic acid (FA) is an important precursor of vanillin which is abundant in cereals like maize, rice and wheat and sugar beet. A major drawback of microbial vanillin production from FA is the degradation and biotransformation of toxic vanillin to other phenolic derivatives. The present study is undertaken to explore microbial vanillin production from FA precursor rice bran by employing vanillin-resistant Pediococcus acidilactici BD16, a natural lactic acid bacteria isolate. Extracellular, intracellular and cellular vanillin dehydrogenase activity was found least, which was minimized vanillin degradation, and the strain resists more than 5 g L−1 vanillin in the medium. A metabolomics approach was followed for the detection of FA, vanillin and other metabolites generated during fermentation of rice bran medium. A metabolic pathway was also predicted for vanillin biosynthesis. Approximately 1.06 g L−1 of crude vanillin was recovered from rice-bran-containing medium and this further offers scope for the industrial utilization of the organism and its genetic manipulation to enhance production of biovanillin.
AB - Owing to its flavoring, antimicrobial, antioxidant and anticarcinogenic nature, vanillin is widely used in foods, beverages, perfumes and pharmaceutical products. Ferulic acid (FA) is an important precursor of vanillin which is abundant in cereals like maize, rice and wheat and sugar beet. A major drawback of microbial vanillin production from FA is the degradation and biotransformation of toxic vanillin to other phenolic derivatives. The present study is undertaken to explore microbial vanillin production from FA precursor rice bran by employing vanillin-resistant Pediococcus acidilactici BD16, a natural lactic acid bacteria isolate. Extracellular, intracellular and cellular vanillin dehydrogenase activity was found least, which was minimized vanillin degradation, and the strain resists more than 5 g L−1 vanillin in the medium. A metabolomics approach was followed for the detection of FA, vanillin and other metabolites generated during fermentation of rice bran medium. A metabolic pathway was also predicted for vanillin biosynthesis. Approximately 1.06 g L−1 of crude vanillin was recovered from rice-bran-containing medium and this further offers scope for the industrial utilization of the organism and its genetic manipulation to enhance production of biovanillin.
KW - ferulic acid esterase
KW - metabolic pathway
KW - Pediococcus acidilactici
KW - phenolic metabolites
KW - rice bran
KW - vanillin
KW - vanillin dehydrogenase
UR - http://www.scopus.com/inward/record.url?scp=84991037013&partnerID=8YFLogxK
U2 - 10.1080/09593330.2016.1237556
DO - 10.1080/09593330.2016.1237556
M3 - Journal article
C2 - 27734757
AN - SCOPUS:84991037013
SN - 0959-3330
VL - 38
SP - 1823
EP - 1834
JO - Environmental Technology (United Kingdom)
JF - Environmental Technology (United Kingdom)
IS - 13-14
ER -