Aerobic and anaerobic ammonium-oxidising bacterial enrichment from municipal solid waste

S. Sri Shalini*, J. Kurian, Jonathan W C WONG

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

4 Citations (Scopus)

Abstract

Leachate from the municipal solid waste (MSW) landfills contains high concentration of ammoniacal nitrogen that is a major toxic pollutant that has a great threat to environment. Among the processes available for the removal of ammoniacal nitrogen, one process is a combination of partial nitrification and anaerobic ammonium oxidation (anammox) process. It requires aerobic ammonium-oxidising bacteria (AOB) and anaerobic ammonium-oxidising bacteria (AnAOB). This paper presents the feasibility of enriching the AOB and AnAOB in 100-mL and 2.5-L batch reactors from fresh and mined MSW and leachate under aerobic and anaerobic conditions with varying feed-to-seed ratio. The AOB and AnAOB activity was monitored by measuring the intermediates such as hydroxylamine and hydrazine along with variations in ammoniacal nitrogen, nitrite nitrogen, and nitrate nitrogen concentrations in the reactor contents. The formation of intermediates such as hydroxylamine and hydrazine and ammoniacal nitrogen transformation data confirmed the enrichment of AOB and AnAOB. Further, AOB and AnAOB were validated by most probable number test and scanning electron microscopy analysis, respectively. DNA extraction, polymerase chain reaction amplification, and sequencing analysis authenticated the AnAOB as Candidatus Brocadia anammoxidans.

Original languageEnglish
Pages (from-to)1107-1122
Number of pages16
JournalInternational Journal of Environmental Science and Technology
Volume12
Issue number3
DOIs
Publication statusPublished - Mar 2015

Scopus Subject Areas

  • Environmental Engineering
  • Environmental Chemistry
  • Agricultural and Biological Sciences(all)

User-Defined Keywords

  • Aerobic ammonium-oxidising bacteria
  • Ammoniacal nitrogen removal
  • Anammox bacteria
  • Bacterial enrichment
  • Candidatus Brocadia anammoxidans
  • Feed-to-seed ratio

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