TY - JOUR
T1 - Aerobic and anaerobic ammonium-oxidising bacterial enrichment from municipal solid waste
AU - Sri Shalini, S.
AU - Kurian, J.
AU - WONG, Jonathan W C
N1 - Publisher Copyright:
© 2014, Islamic Azad University (IAU).
PY - 2015/3
Y1 - 2015/3
N2 - Leachate from the municipal solid waste (MSW) landfills contains high concentration of ammoniacal nitrogen that is a major toxic pollutant that has a great threat to environment. Among the processes available for the removal of ammoniacal nitrogen, one process is a combination of partial nitrification and anaerobic ammonium oxidation (anammox) process. It requires aerobic ammonium-oxidising bacteria (AOB) and anaerobic ammonium-oxidising bacteria (AnAOB). This paper presents the feasibility of enriching the AOB and AnAOB in 100-mL and 2.5-L batch reactors from fresh and mined MSW and leachate under aerobic and anaerobic conditions with varying feed-to-seed ratio. The AOB and AnAOB activity was monitored by measuring the intermediates such as hydroxylamine and hydrazine along with variations in ammoniacal nitrogen, nitrite nitrogen, and nitrate nitrogen concentrations in the reactor contents. The formation of intermediates such as hydroxylamine and hydrazine and ammoniacal nitrogen transformation data confirmed the enrichment of AOB and AnAOB. Further, AOB and AnAOB were validated by most probable number test and scanning electron microscopy analysis, respectively. DNA extraction, polymerase chain reaction amplification, and sequencing analysis authenticated the AnAOB as Candidatus Brocadia anammoxidans.
AB - Leachate from the municipal solid waste (MSW) landfills contains high concentration of ammoniacal nitrogen that is a major toxic pollutant that has a great threat to environment. Among the processes available for the removal of ammoniacal nitrogen, one process is a combination of partial nitrification and anaerobic ammonium oxidation (anammox) process. It requires aerobic ammonium-oxidising bacteria (AOB) and anaerobic ammonium-oxidising bacteria (AnAOB). This paper presents the feasibility of enriching the AOB and AnAOB in 100-mL and 2.5-L batch reactors from fresh and mined MSW and leachate under aerobic and anaerobic conditions with varying feed-to-seed ratio. The AOB and AnAOB activity was monitored by measuring the intermediates such as hydroxylamine and hydrazine along with variations in ammoniacal nitrogen, nitrite nitrogen, and nitrate nitrogen concentrations in the reactor contents. The formation of intermediates such as hydroxylamine and hydrazine and ammoniacal nitrogen transformation data confirmed the enrichment of AOB and AnAOB. Further, AOB and AnAOB were validated by most probable number test and scanning electron microscopy analysis, respectively. DNA extraction, polymerase chain reaction amplification, and sequencing analysis authenticated the AnAOB as Candidatus Brocadia anammoxidans.
KW - Aerobic ammonium-oxidising bacteria
KW - Ammoniacal nitrogen removal
KW - Anammox bacteria
KW - Bacterial enrichment
KW - Candidatus Brocadia anammoxidans
KW - Feed-to-seed ratio
UR - http://www.scopus.com/inward/record.url?scp=84922580604&partnerID=8YFLogxK
U2 - 10.1007/s13762-013-0486-7
DO - 10.1007/s13762-013-0486-7
M3 - Journal article
AN - SCOPUS:84922580604
SN - 1735-1472
VL - 12
SP - 1107
EP - 1122
JO - International Journal of Environmental Science and Technology
JF - International Journal of Environmental Science and Technology
IS - 3
ER -