TY - JOUR
T1 - Adrenomedullin Increases the Short-Circuit Current in the Mouse Seminal Vesicle: Actions on Chloride Secretion
AU - Liao, S.B.
AU - Cheung, K.H.
AU - O, W.S.
AU - Tang, Fai
N1 - Funding information:
This study was substantially supported by a GRF grant from the Hong Kong Research Grant Council (HKU 775908) and a CRCG (Committee on Research and Conference Grants) grant from the University of Hong Kong.
Publishe copyright:
© 2014 by the Society for the Study of Reproduction, Inc.
PY - 2014/8/1
Y1 - 2014/8/1
N2 - Adrenomedullin (ADM) may regulate seminal vesicle fluid secretion, and this may affect sperm quality. In this study, we investigated the effect of ADM on chloride secretion in the mouse seminal vesicle. The presence of ADM in mouse seminal vesicle was confirmed using immunostaining, and the molecular species was determined using gel filtration chromatography coupled with enzyme-linked assay for ADM. The effects of ADM on chloride secretion were studied by short-circuit current technique in a whole-mount preparation of mouse seminal vesicle in an Ussing chamber. The effects of specific ADM and calcitonin gene-related peptide (CGRP) receptor antagonists were investigated. Whether the ADM effect depended on the cAMP- and/or calcium-activated chloride channel was also studied using specific chloride channel blockers. The results showed that ADM was present in seminal vesicle epithelial cells. The major molecular species was precursor in the mouse seminal vesicle. ADM increased short-circuit current through the calcium-activated chloride channel in mouse seminal vesicle, and CGRP receptor was involved. We conclude that ADM may regulate chloride and fluid secretion from the seminal vesicle, which may affect the composition of the seminal plasma bathing the sperm and, hence, fertility.
AB - Adrenomedullin (ADM) may regulate seminal vesicle fluid secretion, and this may affect sperm quality. In this study, we investigated the effect of ADM on chloride secretion in the mouse seminal vesicle. The presence of ADM in mouse seminal vesicle was confirmed using immunostaining, and the molecular species was determined using gel filtration chromatography coupled with enzyme-linked assay for ADM. The effects of ADM on chloride secretion were studied by short-circuit current technique in a whole-mount preparation of mouse seminal vesicle in an Ussing chamber. The effects of specific ADM and calcitonin gene-related peptide (CGRP) receptor antagonists were investigated. Whether the ADM effect depended on the cAMP- and/or calcium-activated chloride channel was also studied using specific chloride channel blockers. The results showed that ADM was present in seminal vesicle epithelial cells. The major molecular species was precursor in the mouse seminal vesicle. ADM increased short-circuit current through the calcium-activated chloride channel in mouse seminal vesicle, and CGRP receptor was involved. We conclude that ADM may regulate chloride and fluid secretion from the seminal vesicle, which may affect the composition of the seminal plasma bathing the sperm and, hence, fertility.
UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-84938941664&partnerID=MN8TOARS
U2 - 10.1095/biolreprod.113.116848
DO - 10.1095/biolreprod.113.116848
M3 - Journal article
SN - 0006-3363
VL - 91
JO - Biology of Reproduction
JF - Biology of Reproduction
IS - 2
M1 - 31
ER -