Activities of starch hydrolytic enzymes and sucrose-phosphate synthase in the stems of rice subjected to water stress during grain filling

Jianchang Yang, Jianhua ZHANG*, Zhiqing Wang, Qingsen Zhu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

123 Citations (Scopus)

Abstract

To understand the effect of water stress on the remobilization of prestored carbon reserves, the changes in the activities of starch hydrolytic enzymes and sucrose-phosphate synthase (SPS) in the stems of rice (Oryza sativa L.) during grain filling were investigated. Two rice cultivars, showing high lodging-resistance and slow remobilization, were grown in the field and subjected to well-watered (WW, ψsoil = 0) and water-stressed (WS, ψsoil = -0.05 MPa) treatments 9 d after anthesis (DAA) till maturity. Leaf water potentials of both cultivars markedly decreased during the day as a result of WS treatment, but completely recovered by early morning. WS treatment accelerated the reduction of starch in the stems, promoted the reallocation of prefixed 14C from the stems to grains, shortened the grain filling period, and increased the grain filling rate. More soluble sugars including sucrose were accumulated in the stems under WS than under WW treatments. Both α- and β-amylase activities were enhanced by the WS, with the former enhanced more than the latter, and were significantly correlated with the concentrations of soluble sugars in the stems. The other two possible starch-breaking enzymes, α-glucosidase and starch phosphorylase, showed no significant differences in the activities between the WW and WS treatments. Water stress also increased the SPS activity that is responsible for sucrose production. Both Vlimit and Vmax, the activities of the enzyme at limiting and saturating substrate concentrations, were enhanced and the activation state (Vlimit/Vmax) was also increased as a result of the more significant enhancement of Vlimit. The enhanced SPS activity was closely correlated with an increase of sucrose accumulation in the stems. The results suggest that the fast hydrolysis of starch and increased carbon remobilization were attributed to the enhanced α-amylase activity and the high activation state of SPS when the rice was subjected to water stress.

Original languageEnglish
Pages (from-to)2169-2179
Number of pages11
JournalJournal of Experimental Botany
Volume52
Issue number364
DOIs
Publication statusPublished - 2001

Scopus Subject Areas

  • Physiology
  • Plant Science

User-Defined Keywords

  • Remobilization
  • Rice
  • Starch hydrolytic enzymes
  • Sucrose-phosphate synthase
  • Water stress

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