Activation of nylon net and its application to a biosensor for determination of glucose in human serum

Caifeng Nan; Yan Zhang; Guomei Zhang; Chuan Dong; Shaomin Shuang; Martin M F CHOI

doi:10.1016/j.enzmictec.2008.12.007

Activation of nylon net and its application to a biosensor for determination of glucose in human serum

Caifeng Nan, Yan Zhang, Guomei Zhang, Chuan Dong^*, Shaomin Shuang, Martin M F CHOI

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

46 Citations (Scopus)

Abstract

A glucose biosensor using a glucose oxidase (GO_x)-immobilized nylon net with glutaraldehyde as cross-linking reagent and an oxygen (O₂) electrode for the determination of glucose has been fabricated. The detection scheme was based on the utilization of dissolved O₂ in oxidation of glucose by the membrane bound GO_x. Crucial factors including O-alkylation temperature, reaction times of nylon net with dimethyl sulfate, l-lysine, and glutaraldehyde, and enzyme loading were examined to determine the optimal enzyme immobilization conditions for the best sensitivity of the developed glucose biosensor. In addition, the effects of pH and concentration of phosphate buffer on the response of the biosensor were studied. The glucose biosensor had a linear range of 18 μM to 1.10 mM with the detection limit of 9.0 μM (S/N = 3) and response time of 80 s. The biosensor exhibited both good operational stability with over 200 measurements and long-term storage stability. The results from this biosensor compared well with those of a commercial glucose assay kit in analyzing human serum glucose samples.

Original language	English
Pages (from-to)	249-253
Number of pages	5
Journal	Enzyme and Microbial Technology
Volume	44
Issue number	5
DOIs	https://doi.org/10.1016/j.enzmictec.2008.12.007
Publication status	Published - 6 May 2009

Scopus Subject Areas

Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology

User-Defined Keywords

Activation
Glucose
Glucose biosensor
Glucose oxidase
Nylon net

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10.1016/j.enzmictec.2008.12.007

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title = "Activation of nylon net and its application to a biosensor for determination of glucose in human serum",

abstract = "A glucose biosensor using a glucose oxidase (GOx)-immobilized nylon net with glutaraldehyde as cross-linking reagent and an oxygen (O2) electrode for the determination of glucose has been fabricated. The detection scheme was based on the utilization of dissolved O2 in oxidation of glucose by the membrane bound GOx. Crucial factors including O-alkylation temperature, reaction times of nylon net with dimethyl sulfate, l-lysine, and glutaraldehyde, and enzyme loading were examined to determine the optimal enzyme immobilization conditions for the best sensitivity of the developed glucose biosensor. In addition, the effects of pH and concentration of phosphate buffer on the response of the biosensor were studied. The glucose biosensor had a linear range of 18 μM to 1.10 mM with the detection limit of 9.0 μM (S/N = 3) and response time of 80 s. The biosensor exhibited both good operational stability with over 200 measurements and long-term storage stability. The results from this biosensor compared well with those of a commercial glucose assay kit in analyzing human serum glucose samples.",

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author = "Caifeng Nan and Yan Zhang and Guomei Zhang and Chuan Dong and Shaomin Shuang and CHOI, {Martin M F}",

note = "Funding Information: The work described in this article was supported by a grant from the Key Project of National Natural Science Foundation of China (No. 50534100). The Graduate Outstanding Innovation Project of Shanxi Province (No. 20081028) is also gratefully acknowledged.",

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AU - Zhang, Guomei

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AU - Shuang, Shaomin

AU - CHOI, Martin M F

N1 - Funding Information: The work described in this article was supported by a grant from the Key Project of National Natural Science Foundation of China (No. 50534100). The Graduate Outstanding Innovation Project of Shanxi Province (No. 20081028) is also gratefully acknowledged.

PY - 2009/5/6

Y1 - 2009/5/6

N2 - A glucose biosensor using a glucose oxidase (GOx)-immobilized nylon net with glutaraldehyde as cross-linking reagent and an oxygen (O2) electrode for the determination of glucose has been fabricated. The detection scheme was based on the utilization of dissolved O2 in oxidation of glucose by the membrane bound GOx. Crucial factors including O-alkylation temperature, reaction times of nylon net with dimethyl sulfate, l-lysine, and glutaraldehyde, and enzyme loading were examined to determine the optimal enzyme immobilization conditions for the best sensitivity of the developed glucose biosensor. In addition, the effects of pH and concentration of phosphate buffer on the response of the biosensor were studied. The glucose biosensor had a linear range of 18 μM to 1.10 mM with the detection limit of 9.0 μM (S/N = 3) and response time of 80 s. The biosensor exhibited both good operational stability with over 200 measurements and long-term storage stability. The results from this biosensor compared well with those of a commercial glucose assay kit in analyzing human serum glucose samples.

AB - A glucose biosensor using a glucose oxidase (GOx)-immobilized nylon net with glutaraldehyde as cross-linking reagent and an oxygen (O2) electrode for the determination of glucose has been fabricated. The detection scheme was based on the utilization of dissolved O2 in oxidation of glucose by the membrane bound GOx. Crucial factors including O-alkylation temperature, reaction times of nylon net with dimethyl sulfate, l-lysine, and glutaraldehyde, and enzyme loading were examined to determine the optimal enzyme immobilization conditions for the best sensitivity of the developed glucose biosensor. In addition, the effects of pH and concentration of phosphate buffer on the response of the biosensor were studied. The glucose biosensor had a linear range of 18 μM to 1.10 mM with the detection limit of 9.0 μM (S/N = 3) and response time of 80 s. The biosensor exhibited both good operational stability with over 200 measurements and long-term storage stability. The results from this biosensor compared well with those of a commercial glucose assay kit in analyzing human serum glucose samples.

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Activation of nylon net and its application to a biosensor for determination of glucose in human serum

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