TY - JOUR
T1 - Acetylcholinesterase inhibitors from Corydalis yanhusuo
AU - XIAO, Haitao
AU - Peng, Jiao
AU - Liang, Yan
AU - Yang, Jie
AU - Bai, Xue
AU - Hao, Xiao Yan
AU - Yang, Fu Mei
AU - Sun, Qian Yun
N1 - Funding Information:
This work was supported by grants from the Natural Science Foundation of Guizhou Province (Grant Nos. 20052068 and 20033048), and the Special Fund for Excellent Scholars in Education, Science and Technology of the Guizhou Provincial Governor.
PY - 2011/9
Y1 - 2011/9
N2 - In a bioassay-guided search for acetylcholinesterase (AChE) inhibitors from Chinese natural resources, eight isoquinoline alkaloids, tetrahydropalmatine (1), corydaline (2), protopine (3), berberine (4), palmatine (5), jatrorrhizine (6), coptisine (7) and dehydrocorydaline (8), were isolated from the methanolic extract of the tubers of Corydalis yanhusuo. Structures of these compounds were identified by spectroscopic techniques. Compounds 4-8 inhibited AChE activity in a dose-dependent manner, and the IC 50 values were 0.47±0.01, 0.74±0.06, 2.08±0.09, 1.01±0.03 and 0.62±0.05μ M, respectively. Structure-activity relationship analysis suggested that aromatisation at ring C, as well as substitutions at C-2, C-3, C-9, C-10 and C-13 affect the AChE activity of protoberberine alkaloids.
AB - In a bioassay-guided search for acetylcholinesterase (AChE) inhibitors from Chinese natural resources, eight isoquinoline alkaloids, tetrahydropalmatine (1), corydaline (2), protopine (3), berberine (4), palmatine (5), jatrorrhizine (6), coptisine (7) and dehydrocorydaline (8), were isolated from the methanolic extract of the tubers of Corydalis yanhusuo. Structures of these compounds were identified by spectroscopic techniques. Compounds 4-8 inhibited AChE activity in a dose-dependent manner, and the IC 50 values were 0.47±0.01, 0.74±0.06, 2.08±0.09, 1.01±0.03 and 0.62±0.05μ M, respectively. Structure-activity relationship analysis suggested that aromatisation at ring C, as well as substitutions at C-2, C-3, C-9, C-10 and C-13 affect the AChE activity of protoberberine alkaloids.
KW - Corydalis yanhusuo; acetylcholinesterase
KW - isoquinoline alkaloids
UR - http://www.scopus.com/inward/record.url?scp=80052976302&partnerID=8YFLogxK
U2 - 10.1080/14786410802496911
DO - 10.1080/14786410802496911
M3 - Journal article
C2 - 20234973
AN - SCOPUS:80052976302
SN - 1478-6419
VL - 25
SP - 1418
EP - 1422
JO - Natural Product Research
JF - Natural Product Research
IS - 15
ER -